Advances in Rodent Housing and Management: Optimizing Conditions for Medical Research Excellence

Merilync Tesalonika, Naula Jamal Munabari, Nany Hairunisa, Nor Azlina Khalil, Rodiah Mohd Radzi

Abstract

Objective: Housing and management of experimental animals are important aspects to consider alongside the welfare of experimental animals. Both are related to the behavior of animals and the results of the research to be conducted. Every laboratorian should pay attention to the management and housing of experimental animals, starting from the characteristics of cages according to the needs of the animals, sanitation that must be considered, and cage enrichment so that animals can express their natural behaviors. The focus on good management and housing aims to reduce stress on animals, create comfort and optimal care, and ensure more accurate research results.

Methods: This article is based on scientific information obtained from various literature such as articles and books published from various sources in the last 10 years from 2014-2024. Data collection was carried out by searching for scientific articles and journals using the Google Scholar search engine containing the concepts being studied. Article searches were carried out using the keywords "Characteristics of Animal Housing", "Management of experimental rodents", and "Rodentia" so that the articles that appeared matched the research topic.

Results: providing suitable living conditions enables animals to develop, reach maturity, reproduce, and exhibit normal behavior in both laboratory and healthy environments. Conclusions: Social animals such as rodents thrive when they have companionship. Mice and rats are active during the night, while guinea pigs are active in daylight. Cage size is adjusted to the needs of each animal so that they can engage in their natural activities. Room temperature is also considered to avoid problems for the animals. Environmental enrichment for rodent animals needs to be considered to minimize stress.

 

Keywords: rodent animals; animal management; cages; housing; characteristics of animal homes

Keywords

rodent animals; animal management; cages; housing; characteristics of animal homes

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References

  1. Neff, E.P. 2021. Rats are on the rise. Lab Anim (NY) 50:205–208. Doi: 10.1038/s41684-021-00812-0
  2. Daniali M, and Abdollabi M. 2023. Animal and computational models in toxicology and pharmacology. Encyclopedia of Toxicology Fourth Edition 1:489–494. Doi: 10.1016/B978-0-12-824315-2.00038-5
  3. Hickman, D.L., Hickman-Davis, J.M., Peveler, J., and Swan, M. 2018 Management of Animal Care and Use Programs in Research, Education, and Testing, 2nd ed. CRC Press / Taylor & Francis, Boca Raton, Florida
  4. Cait, J, Cait, A, Scott, R.W., Winder, C.B., and Mason, G.J. 2022. Conventional laboratory housing increases morbidity and mortality in research rodents: results of a meta-analysis. BMC Biol. https://doi.org/10.1186/s12915-021-01184-0
  5. Delaney, M.A., Treuting, P.M., and Rothenburger, J.L. 2018. Rodentia. Pathology of Wildlife and Zoo Animals 499–515. Doi: 10.1016/B978-0-12-805306-5.00020-1
  6. Moreno-Jiménez, E.P., Jurado-Arjona, J., Avila, J., and Martín, M.L. 2019. The social component of environmental enrichment is a pro-neurogenic stimulus in adult c57BL6 female mice. Front Cell Dev Biol. https://doi.org/10.3389/fcell.2019.00062
  7. Prepared by the Animal Facilities Standards Committee of the Animal Care Panel. 2021. Guide for Laboratory Animal Facilities and Care. ILAR J 62:345–358. doi: 10.1093/ilar/ilac012
  8. Lidster, K., Owen, K., Browne, W.J., and Prescott, M.J. 2019. Cage aggression in group-housed laboratory male mice: an international data crowdsourcing project. Sci Rep. https://doi.org/10.1038/s41598-019-51674-z
  9. Kappel, S., Hawkins, P., and Mendl, M.T. 2017 To group or not to group? Good practice for housing male laboratory mice. Animals. https://doi.org/10.3390/ani7120088

10. Kim, Y.H., and Lee, J.Y. 2023. Implementation of an Individually Ventilated Cage System Applying Air Conditioning Control Method. 한국컴퓨터정보학회논문지 Journal of The Korea Society of Computer and Information 28:1–12. Doi: 10.9708/jksci.2023.28.06.001

11. Stover, M.G., and Villano, J.S. 2022. Evaluation of Various IVC Systems According to Mouse Reproductive Performance and Husbandry and Environmental Parameters. Journal of the American Association for Laboratory Animal Science 61:31–41. Doi: 10.30802/AALAS-JAALAS-21-000079

12. Makowska, I.J., Franks, B., El-Hinn, C., Jorgensen, T., and Weary, D.M. 2019. Standard laboratory housing for mice restricts their ability to segregate space into clean and dirty areas. Sci Rep. https://doi.org/10.1038/s41598-019-42512-3

13. Jackson, E., Demarest, K., Eckert, W.J., Cates-Gatto, C., Nadav, T., Cates, L.N., et al. 2015. Aspen shaving versus chip bedding: Effects on breeding and behavior. Lab Anim 49:46–56. Doi: 10.1177/0023677214553320

14. Home Office. 2014. Mice, rats, gerbils, hamsters and guinea pigs. In: Code of practice for the housing and care of animals bred, supplied or used for scientific purposes. Her Majesty’s Stationery Office.

15. Cloutier, S., Wahl, K., Baker, C., and Newberry, R.C. 2014. The Social Buffering Effect of Playful Handling on Responses to Repeated Intraperitoneal Injections in Laboratory Rats. Journal of the American Association for Laboratory Animal Science 53:161–166. PMID: 24602543; PMCID: PMC3966273.

16. Peirson, S.N., Brown, L.A., Pothecary, C.A., Benson, L.A., and Fisk, A.S. 2018. Light and the laboratory mouse. J Neurosci Methods 300:26–36. Doi: 10.1016/j.jneumeth.2017.04.007

17. Beery, A.K., and Kaufer, D. 2015. Stress, social behavior, and resilience: Insights from rodents. Neurobiol Stress 1:116–127. Doi: 10.1016/j.ynstr.2014.10.004

18. Golledge, H. 2016. Comfortable Quarters For Laboratory Animals, 10th ed. Animal Welfare Institute, Washington DC. Doi: 10.1017/S0962728600007922

19. Škop, V., Guo, J., Liu, N., Xiao, C., Hall, K.D., Gavrilova, O., et al. 2020. Mouse Thermoregulation: Introducing the Concept of the Thermoneutral Point. Cell Rep. https://doi.org/10.1016/j.celrep.2020.03.065

20. Leidinger, C.S., Thöne-Reineke, C., Baumgart, N., and Baumgart, J. 2019. Environmental enrichment prevents pup mortality in laboratory mice. Lab Anim 53:53–62. Doi: 10.1177/0023677218777536

21. Mutiarahmi, C.N., Hartady, T., and Lesmana, R. 2021. Use Of Mice As Experimental Animals In Laboratories That Refer To The Principles Of Animal Welfare: A Literature Review. Indonesia Medicus Veterinus 10:134–145. Doi: 10.19087/imv.2020.10.1.134

22. Ratuski, A.S., and Weary, D.M. 2022. Environmental Enrichment for Rats and Mice Housed in Laboratories: A Metareview. Animals. https://doi.org/10.3390/ani12040414

23. Modlinska, K., and Pisula, W. 2020. The natural history of model organisms the Norway rat, from an obnoxious pest to a laboratory pet. Elife. https://doi.org/10.7554/eLife.50651

24. Hawkins, P., and Golledge, H.D. 2018. The 9 to 5 Rodent − Time for Change? Scientific and animal welfare implications of circadian and light effects on laboratory mice and rats. J Neurosci Methods 300:20–25. Doi: 10.1016/j.jneumeth.2017.05.014

25. Kalueff, A.V., Stewart, A.M., Song, C., Berridge, K.C., Graybiel, A.M., and Fentress, J.C. 2016. Neurobiology of rodent self-grooming and its value for translational


neuroscience. Nat Rev Neurosci 17:45–59. Doi: 10.1038/nrn.2015.8

26. Inagaki, H., Kiyokawa, Y., Tamogami, S., Watanabe, H., Takeuchi, Y., and Mori, Y. 2014. Identification of a pheromone that increases anxiety in rats. Proc Natl Acad Sci U S A. 111:18751–18756. Doi: 10.1073/pnas.1414710112

27. Patki, G., Atrooz, F., Alkadhi, I., Solanki, N., and Salim, S. 2015. High aggression in rats is associated with elevated stress, anxiety-like behavior, and altered catecholamine content in the brain. Neurosci Lett 308–313. Doi: 10.1016/j.neulet.2014.10.051

28. Modlinska, K., Stryjek, R., and Pisula, W. 2015. Food neophobia in wild and laboratory rats (multi-strain comparison). Behavioural Processes 113:41–50. Doi: 10.1016/j.beproc.2014.12.005

29. Leidinger, C.S., Kaiser, N., Baumgart, N., and Baumgart, Jan. 2018. Using Clicker Training and Social Observation to Teach Rats to Voluntarily Change Cages. J Vis Exp. Doi. 10.3791/58511

30. Makowska, I.J., and Weary, D.M. 2016. The importance of burrowing, climbing and standing upright for laboratory rats. R Soc Open Sci. https://doi.org/10.1098/rsos.160136

31. Skinner, M., Ceuppens, P., White, P., and Prior, H. 2019. Social-housing and use of double-decker cages in rat telemetry studies. J Pharmacol Toxicol Methods 96:87–94. Doi: 10.1016/j.vascn.2019.02.005

32. Wheeler, R.R., Swan, M.P., and Hickman, D.L. 2015. Effect of multilevel laboratory rat caging system on the well-being of the singly-housed sprague dawley rat. Lab Anim 49:10–19. Doi: 10.1177/0023677214547404

33. Polosa, A., Bessaklia, H., and Lachapelle, P. 2016. Strain Differences in Light-Induced Retinopathy. PLoS One. 1(6). Doi: 10.1371/journal.pone.0158082

34. Vogt, M.A., Geiger, L.M.J., Härtel, T., Follert, P., Palme, R., and Chourbaji, S. 2021. Evaluation of potential sustainable bedding substrates focusing on preference, behavior, and stress physiology in rats—a pilot study. Animals. https://doi.org/10.3390/ani11051375

35. Harland, B.C., and Alford, J.C.D. 2020. Enriched Environment Procedures for Rodents: Creating a Standardized Protocol for Diverse Enrichment to Improve Consistency across Research Studies. Bio Protocol. 10 (11). Doi: 10.21769/BioProtoc.3637

36. Abou-Ismail, U.A., Darwish, R.A., and Ramadan, S.G.A. 2014. Should Cages of Laboratory Rats Be Enriched Physically or Socially?. Glob Vet. 13:570–582. Doii: 10.5829/idosi.gv.2014.13.04.85237

37. Modlinska, K., Chrzanowska, A., and Pisula, W. 2019. The impact of changeability of enriched environment on exploration in rats. Behavioural Processes 164:78–85. Doi: 10.1016/j.beproc.2019.04.015

38. Ratuski, A.S., and Weary, D.M. 2021. A break from the pups: The effects of loft access on the welfare of lactating laboratory rats. PLoS One. https://doi.org/10.1371/journal.pone.0253020

39. Hinchcliffe, J.K., Jackson, M.G., and Robinson, E.S.J. 2022. The use of ball pits and playpens in laboratory Lister Hooded male rats induces ultrasonic vocalisations indicating a more positive affective state and can reduce the welfare impacts of aversive procedures. Lab Anim 56:370–379. Doi: 10.1177/00236772211065920

40. Clemons, D.J., and Seeman, J.L. 2018 The Laboratory Guinea Pig. Boca Raton. 2 ed. Doi: https://doi.org/10.1201/b12886

41. Donnelly, M. 2015. Guinea pigs. In: Comfortable Quarters for Laboratory Animals, 10th ed. Animal Welfare Institute.

42. Shomer, N.H., Holcombe, H., and Harkness, J.E. 2015. Biology and Diseases of Guinea Pigs. In: Laboratory Animal Medicine: Third Edition. Elsevier Inc., pp 247–283. Doi: 10.1016/B978-0-12-409527-4.00006-7

43. Balaguer, C.M., Capilla, A.C., Delgado, V.A.Z., and Macías, D.S. 2019. A comparison of the growth performance, carcass traits, and behavior of guinea pigs reared in wire cages and floor pens for meat production. Meat Sci 152:38–40. Doi: 10.1016/j.meatsci.2019.02.012

44. Quesenberry, K.E. and Donnelly, T.M.2020. Providing a home for mice. https://www.merckvetmanual.com/all-other-pets/mice/providing-a-home-for-mice.

45. Canadian Council on Animal Care Conseil canadien de protection des animaux. 2019. Canadian Council on Animal Care. https://ccac.ca/Documents/Standards/Guidelines/CCAC_Guidelines_Mice.pdf.

46. Kucic, M. 2023. The best mice cages for an ethical living environment. https://www.animallama.com/mice/best-mouse-cages/

47. Du, D. (2021) How big should a rat cage be? A rat cage size guide. https://squeaksandnibbles.com/rat-cage-size/

48. C&C Guinea Pig Cages. 2024. Gallery - C&C Guinea Pig Cages - Mesh and Grid Cages for Pets. https://candcguineapigcages.co.uk/gallery/

DOI: https://doi.org/10.20961/lar.v22i3.84171

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