Quantitative characteristics and growth hormone gene diversity of thin-tailed sheep in Sitinjau Laut, Kerinci Regency

Rhaphon Seprian. Ht, Depison Depison, Eko Wiyanto

Abstract

Objective: This study aims to evaluate the quantitative characteristics and growth hormone gene diversity of thin-tailed sheep in Sitinjau Laut, Kerinci Regency.

Methods: The research method used 60 thin-tailed sheep and 60 samples of thin-tailed sheep's blood. The phenotypes observed included: body weight, weight gain, body measurements, and thin-tailed sheep blood samples. The GH gene was identified using the PCR-RFLP method with the Msp1 restriction enzyme. Data analysis included t-test, t2-hotelling, principal component analysis, and allele genotype frequencies.

Results: The results showed that body weight, body weight gain, and sizes of male thin-tailed sheep were significantly different (P<0.05) higher than females. The analysis results on the GH|Msp1 gene locus of thin-tailed sheep were monomorphic with one type of allele, namely ++.

Conclusions: The average body weight, weight gain, and body measurements of male thin-tail sheep were higher than that of females. The body size characteristic of male and female thin-tailed sheep is the chest circumference, while the body shape characteristic of male and female thin-tailed sheep is the chest depth. The fragmentation of the GH|Msp1 gene in thin-tailed sheep is monomorphic.

Keywords

Growth hormone gene; Thin-tailed sheep; Quantitative characteristics

Full Text:

PDF

References

  1. Najmuddin, M., and M. Nasich. 2019. Produktivitas induk domba ekor tipis di Desa Sedan Kecamatan Sedan Kabupaten Rembang. J. Trop. Anim. Prod. 20:76–83. Doi: 10.21776/ub.jtapro.2019.020.01.10
  2. Komariah, D. J. Setyono, and A. Aslimah. 2015. Karakteristik kuantitatif dan kualitatif kambing dan domba sebagai hewan qurban di mitra tani farm. Bul. Peternak. 39:84–91. Doi: 10.21059/buletin-peternak.v39i2.6712
  3. Depison, A. Sarbaini, Jamsari, Arnim, and Yurnalis. 2017. Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province. African J. Biotechnol. 16:1159–1167. Doi: 10.5897/ajb2016.15783
  4. Depison, W. P. B. Putra, Gushairiyanto, Y. Alwi, and H. Suryani. 2021. Morphometrics characterization of Thin-Tail sheep in lowland and highland areas. Trop. Anim. Sci. J. 44:386–398. Doi: 10.5398/tasj.2021.44.4.386
  5. Yurnalis, Y., A. Arnim, S. Sarbaini, and J. Jamsari. 2017. Keragaman baru pada daerah ujung gen hormon pertumbuhan sapi pesisir ternak lokal Sumatera Barat. J. Peternak. Indones. 19:107–113. Doi: 10.-25077/jpi.19.3.103-109.2017
  6. Hartatik, T., D. E. Putra, S. D. Volkandari, T. Kanazawa, and S. Sumadi. 2018. Genotype analysis of partial growth hormone gene (GH891MspI) in pesisir cattle and simmental-pesisir crossbred cattle. J. Indones. Trop. Anim. Agric. 43:1–8. Doi: 10.14710/jitaa.43.1.1-8
  7. Trasia, R. F. 2020. Nested polymerase chain reaction (Nested-PCR) as a diagnostic technique for intestinal helminth infection. J. Al-Azhar Indones. 5:183–185. Doi: 10.36722/sst.v5i4.427
  8. Anggraini, A. D., E. B. Koendhori, H. Pramono, and D. D. Wahyono. 2017. Polymorphism analysis of the coagulase gene in isolates of methicillin-resistant staphylococcus aureus with AluI restriction sites. Heal. Sci. J. Indones. 8:19–24. Doi: 10.22435/hsji.v8i1.6865.1-6
  9. Ewida, R. M. and D. S. M. Abd El-Magiud. 2018. Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism. Vet. World. 11:830–833. Doi: 10.14202/-vetworld.2018.830-833
  10. Gaspersz, V. 2006. Teknik analisis dalam penelitian percobaan. Penerbit Tarsito, Bandung.
  11. Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press, New York.
  12. Haryanti, Y., E. Kurnianto, and C. M. S. Lestari. 2015. Pendugaan bobot badan menggunakan ukuran-ukuran tubuh pada domba Wonosobo. J. Sain Peternak. Indones. 10:1–6. Doi: 10.31186/jspi.id.10.-1.1-6
  13. Yantoro, A. D., W. Wardoyo, and R. K. Dewi. 2020. Identifikasi sifat kuantitatif dan kualitatif domba lokal di Desa Jotosanur Kecamatan Tikung Kabupaten Lamongan. Int. J. Anim. Sci. 3:124–128. Doi: 10.30736/ijasc.v3i04.29
  14. Wattimena, J., J. Labentubun, and M. Matatula. 2014. Sifat-sifat kuantitatif dan kualitatif domba Kisar jantan. Agrinimal J. Ilmu Ternak dan Tanam. 4:72–76.
  15. Nurfaridah, A., S. B. Komar, and S. Nurachma. 2012. Indeks kumulatif ukuran-ukuran tubuh dan bobot badan domba komposit betina dewasa sebagai domba pedaging (studi kasus di kandang percobaan Fakultas Peternakan Universitas Padjadjaran). Students e-Journal.
  16. Rachman, R. N., D. Rahmat, B. Ayuningsih, F. T. Santoso, T. Dhalika, and I. Hernaman. 2021. Kurva pertambahan bobot badan domba Garut jantan 13-16 bulan diberi 80% hijauan dan 20% konsentrat. Jurnal Peternakan. 18:122–128. Doi: 10.31602/-zmip.v47i1.5360
  17. Ahmad, I., B. Mukwakhid, and U. Ali. 2023. Pengaruh tingkat penggunaan ampas bir terfermentasi aspergillus niger terhadap kosumsi pakan pertambahan bobot badan dan konversi pakan pada domba lokal. J. Din. rekasatwa. 6:52–58.
  18. Gebreyowhens, W., M. Regesa, and A. Esifanos. 2017. Improving live body weight gain of local sheep through crossbreeding with high yielding exotic Dorper sheep under smallholder farmers. Int. J. Livest. Prod. 8:69–71. Doi: 10.5897/ijlp2016.0316
  19. Khanza, K. N., G. Gushariyanto, and D. Depison. 2021. Hubungan antara karakteristik telur dengan bobot telur dan bobot Day Old Duck (DOD) dengan bobot badan itik Kerinci pada berbagai tingkat umur. J. Ilmu dan Ind. Peternak. 7:159–174.
  20. Ashari, M., R. R. A. Suhardiani, and R. Andriati. 2015. Tampilan bobot badan dan ukuran linier tubuh domba Ekor Gemuk pada umur tertentu di Kabupaten Lombok Timur. J. Ilmu dan Teknol. Peternak. Indones. 1:24–30.
  21. Ananda, M. K., P. Sampurna, and T. S. Nindhia. 2020. Pendugaan bobot karkas sapi bali jantan dan betina berdasarkan panjang badan dan lingkar dada. Indones. Med. Veterinus. 9:512–521. Doi: 10.19087/-imv.2020.9.4.512
  22. Subhandiawan, H., S. B. Komar, and N. Suwarno. 2016. Persamaan laju pertumbuhan domba lokal jantan dan betina umur 1-12 bulan yang ditinjau dari panjang badan dan tinggi pundak. Students e-Journals. 1–13.
  23. Putri, A. B. S. R. N., G. Gushairiyanto, and D. Depison. 2020. Bobot badan dan karakteristik morfometrik beberapa galur ayam lokal. J. Ilmu dan Teknol. Peternak. Trop. 7:256–264. Doi: 10.33772/jitro.v7i3.-1215
  24. Hikmawaty, A. Gunawan, R. Noor, and J. Jakaria. 2014. Identifikasi ukuran tubuh dan bentuk tubuh sapi Bali di beberapa pusat pembibitan melalui pendekatan analisis komponen utama. J. Ilmu Produksi dan Teknol. Has. Peternak. 02:231–237.
  25. Rohayati, T. and E. Herawati. 2020. Identifikasi ukuran-ukuran tubuh domba Garut rancabango Kabupaten Garut. J. Ilmu Peternak. 4:47–53.
  26. Malewa, A. D. and S. Salmin. 2008. Karakteristik domba lokal Palu. J. Agrol. 15:68–74.
  27. Sahrani, I., L. O. Nafiu, and R. Badaruddin. 2022. Korelasi antara bobot badan dan ukuran-ukuran tubuh kambing kacang di Kota Kendari. J. Ilm. Peternak. Halu Oleo. 4:177–183. Doi: 10.56625/jipho.v4i3.27016
  28. Rosiana, I. W. and I. G. Widhiantara. 2018. Optimalisasi produk PCR (Polymerase Chain Reaction) pada analisa keragaman genetik mikrosatelit burung Kakatua Kecil Jambul Kuning (Cacatua sulphurea). J. Media Sains. 2:37–42.
  29. Malewa, A. D. 2019. Keragaman gen hormon pertumbuhan domba Palu dengan metode PCR-RFLP. J. AgriSains. 3:144–153.
  30. Fastawa, R., C. Sumantri, A. Gunawan, and S. Murtini. 2019. Pemberian anti-myostatin pada induk serta keragaman gen myostatin dan asosiasinya terhadap potongan karkas anak ayam Sentul (F1). J. Ilmu Produksi dan Teknol. Has. Peternak. 7:22–28. Doi: 10.29244/jipthp.7.1.22-28

DOI: https://doi.org/10.20961/lar.v22i1.73212

Refbacks

  • There are currently no refbacks.