Computer aided semen analysis (CASA) to determine the quality and fertility of frozen thawed sumba ongole sperm supplemented with amino acids

Tulus Maulana, Paskah Partogi Agung, Muhammad Gunawan, Syahruddin Said

Abstract

Objective: The objective of this research was to investigate the quality and reproductive potential of frozen-thawed Sumba Ongole (SO) bull spermatozoa supplemented with specified amino acids using computer-assisted semen analysis (CASA).

Methods: A total of 18 ejaculates were collected once a week from three Sumba Ongole bulls and diluted in tris-citric-fructose-egg yolk (TCFY) extender and various concentrations of selected amino acids. The post-thaw spermatozoa were examined for motility, head behavior, and swimming pattern using CASA technique.  Data were analysis using SPSS 24 ebvaluated by ANOVA.

Results: Total motility (72.56%±3.20) and progressive motility (71.11%±3.31) the addition of Glutamine 5% significantly different (p<0.05) than other treatments. The addition of 7mM cysteine resulted in significantly (p<0.05) in velocity average path (VAP), velocity curve line (VCL), velocity straight line(VSL) parameters also have higher in distance average path (DAP), distance curve line (DCL), dan distance straight line (DSL) parameters significantly (p<0,05).  

Conclusion: In conclusion, the introduction of CASA systems enabled quantification of individual spermatozoa motility patterns and CASA colud be used to estimate the fertilizing capacity of sperm based on the characteristics of sperm velocity.

Keywords

CASA; Cryopreservation; Fertility; Spermatozoa; Sumba Ongole

Full Text:

PDF

References

  1. Putra, W. P. B., P. P. Agung, and S. Said. 2018. Non-genetic factor and genetic parameter analysis for growth traits in Sumba Ongole (SO) cattle. J. Indones. Trop. Anim. Agric. 43:94-106. Doi: 10.14710/jitaa.43.2.94-106
  2. Ugur, M. R., A. S. Abdelrahman, H. C. Evans, A. A. Gilmore, M. Hitit, R. I. Arifiantini, B. Purwantara, A. Kaya, and E. Memili. 2019. Advances in cryopreservation of bull sperm. Front. Vet. Sci. 6:268. Doi: 10.3389/fvets.2019.00268
  3. Nowicka, K. and B. Nixon. 2020. Molecular changes induced by oxidative stress that impair human sperm motility. Antioxidants. 9:134. Doi: 10.3390/antiox9020134
  4. Zhu, Z., Z. Ren, X. Fan,Y. Pan, S. Lv, and C. Pan. 2017. Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages. PLoS ONE. 12:181-110. Doi: 10.1371/journal.pone.0181110
  5. Topraggaleh, T. R., A. Shahverdi, A. Rastegarnia, B. Ebrahimi, V. Shafiepour, and M. Sharbatoghli. 2014. Effect of cysteine and glutamine added to extender on post-thaw sperm functional parameters of buffalo bull. Andrologia. 46:77-83. Doi: 10.1111/and.12148
  6. Zhu, Z., X. Fan, Y. Lv, Y. Lin, D. Wu, and W. Zeng. 2017. Glutamine protects rabbit spermatozoa against oxidative stress via glutathione synthesis during cryopreservation. Rep. Fert. Develop. 29:2183-2194. Doi: 10.1071/RD17020
  7. Said, S., Setiorini, M. Adella, I. Sari, N. Fathaniah, and T. Maulana. 2019. The effect of addition selected amino acids in extender semen on quality and dna stability of frozen-thawed sumba ongole bull spermatozoa. Indones. J. Anim. Vet. Sci. 24:9-14. Doi: 10.14334/jitv.v24i1.1873
  8. Valverde A., V. Barquero, and C. Soler. 2020. The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review. J. Anim. Feed Sci. 29: 189-198. Doi: 10.22358/jafs/127691/2020
  9. Amann, R. P. and D. Waberski. 2014. Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology. 81:5-17. Doi: 10.1016/j.theriogenology.2013.09.004
  10. Galmessa, U., T. K. Mohanty, V. S. Raina, A. K. Gupta, and S. Prasad. 2014. Post-thawed and fresh spermatozoa motion characteristics of Sahiwal bulls under computer-assisted semen analyser (CASA). Int. J. Livest. Prod. 5:65-70. Doi: 10.5897/IJLP2013.0184
  11. Nagy, A., T. Polichronopoulos, L. Solti, and S. Cseh. 2015. Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Veterinaria Hungarica. 63:370-381. Doi: 10.1556/4.2015.035
  12. INS. 2017. SNI 4869-1:2017 Frozen semen – Part 1: Bovine bull (in Bahasa Indonesia). Indonesian National Standardized Agency. Jakarta, Indonesia.
  13. Said, S., F. Afiati, and T. Maulana. 2015. Study on changes of sperm head morphometry and DNA integrity of freeze-dried bovine spermatozoa. J. Indonesian Trop. Anim. Agric. 40:145-152. Doi: 10.14710/JITAA.40.3.145-152
  14. Forero-Gonzalez, R. A., E. C. C. Celeghini, C. F. Raphael, A. F. C. Andrade, F. F. Bressan, and P. P. Arruda. 2012. Effect of bovine sperm cryopreservation using different freezing techniques and cryoprotective agents on plasma, acrosomal and mitochondrial membranes. Andrologica. 44:154-159. Doi: 10.1016/j.anireprosci.2007.02.001
  15. Keskin, N., C. Erdogan, M. Bucak, A. Öztürk, M. Bodu, P. Ili, N. Baspinar, and S. Dursun. 2020. Cryopreservation Effects on Ram Sperm Ultrastructure. Biopreservation and Biobanking. 18. Doi: 10.1089/bio.2020.0056
  16. Farshad, A. and Y. Hosseini. 2013. The cryoprotective effects of amino acids supplementation on cooled and post- thaw Markhoz bucks semen quality. Small Rumin Res. 114:258-263. Doi: 10.1016/j.smallrumres.2013.07.007
  17. Sariozkan, S., S. Ozdamar, G. Turk, F. Canturk, and A. Yay. 2014. In-vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage. Cryobiology. 68:349-353.
  18. El-Sheshtawy, R. I., G. A. El-Sisy, and W. S. El-Nattat. 2008. Use of selected amino acids to improve buffalo bull semen cryopreservation. Glob. Vet. 2:146-150.
  19. Broekhuijse, M. L., E. Sostaric, H. Feitsma, and B. M. Gadella. 2011. Additional value of com- puter assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination. Theriogenology.76:1473–1486.
  20. Amal, A. S., R. I. Arifiantini, M. A. Setiadi, and S. Said. 2019. Characteristics of the postthawed Balinese bull semen extended in three different extenders and equilibration times. J. Indonesian Trop. Anim. Agric. 44:135-145.
  21. Singh, A. K., P. S. Brar, and R. S. Cheema. 2016. Relationship between sperm penetration distance in cervical mucus and frozen semen characteristics vis-à-vis buffalo bull fertility. Indian. J. Anim. Sci. 86:1405.
  22. Singh, A. K., P. S. Brar, and R. S. Cheema. 2020. Heparin binding proteins in seminal plasma of breeding buffalo bulls and their relation with semen freezability and in vivo fertility. Indian. J. Anim. Sci. 90:41–45.
  23. Kathiravan, P., J. Kalatharan, G. Karthikeya, K. Rengarajan, G. Kadirve. 2011. Objective sperm motion analysis to assess dairy bull fertility using computer-aided system – a review. Reprod. Dom. Anim. 46:165-172.
  24. Oliveira, L. Z., R. P. de Arruda , A. F. C. de Andrade, and E. C. C. Celeghini. 2013. Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program. Anim. Reprod. Sci. 137:145-155.

DOI: https://doi.org/10.20961/lar.v20i2.58754

Refbacks

  • There are currently no refbacks.