Potensi Daun Keji beling (Strobilanthus crispus) sebagai Antibakteri dan Antibiofilm terhadap Bakteri Cutibacterium acnes

Sumi Wijaya, Inggar Dwi Kuncahyani, Lisa Soegianto

Abstract

Cutibacterium acnes, merupakan salah satu mikroorganisme permanen pada permukaan stratum korneum, dimana populasinya mewakili 20-70% dari mikroorganisme yang banyak ditemukan pada kondisi muka berjerawat. Pembentukan biofilm dari mikroorganisme ini merupakan salah satu faktor yang mengakibatkan obat anti jerawat tidak efektif dalam bekerja. Strobilanthus crispus (keji beling) merupakan salah satu tanaman yang memiliki potensi cukup tinggi dalam pengobatan tradisional, khususnya sebagai antibakteri. Penelitian ini akan menguji kemampuan ekstrak etanol daun keji beling sebagai antibakteri dan antibiofilm terhadap bakteri Cutibacterium acnes. Ekstrak etanol daun keji beling di dapatkan dengan menggunakan metode maserasi dengan pelarut etanol 96%. Metode Difusi Sumuran dengan Diameter Hambat Pertumbuhan (DHP) sebagai parameter pengukuran digunakan sebagai metode pengujian untuk aktivitas antibakteri. Ekstrak etanol daun keji beling pada konsentrasi 50% memberikan DHP 7,21 ± 0,103 mm. Aktivitas antibiofilm diuji dengan menggunakan metode mikrodilusi pada rentang konsentrasi ekstrak 0,06 % - 30%. Hasil menunjukkan 1,88% ekstrak etanol daun keji beling telah mampu memberikan persentase penghambatan pembentukan biofilm sebesar 98,23 ± 0,230%, dimana hasil ini menunjukkan hasil yang tidak berbeda dengan klindamisin 10µg/100µL. Skrinning fitokimia yang dilakukan pada ekstrak etanol daun keji beling menunjukkan adanya flavonoid, alkaloid dan tanin, yang di duga menunjukkan potensi sebagai antibiofilm.

Keywords

Strobilanthus crispus; Cutibacterium acnes; Antibakteri; Antibiofilm

References

Adriana, L., Dewi, C., & Nasir, N. H. (2023). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Keji beling (Strobilanthes crispa BI) Terhadap Pertumbuhan Bakteri Staphylococcus epidermidis dan Pseudomonas aeruginosa. Jurnal Pharmacia Mandala Waluya, 2(3), Article 3. https://doi.org/10.54883/jpmw.v2i3.82

Ahmed, M. E., Ahmed, Z. M., & Thamer, A. (2020). The Evolutionary Effects of Bacillin and S-Pyocin Bacteriocin and Their Effects on Propionibacterium acnes and Fungi. Biochem. Cell. Arch., 20(2), 3645–3649. https://www.researchgate.net/publication/344616801

Alibi, S., Crespo, D., & Navas, J. (2021). Plant-Derivatives Small Molecules with Antibacterial Activity. Antibiotics, 10(3), 231. https://doi.org/10.3390/antibiotics10030231

Coenye, T., Spittaels, K.-J., & Achermann, Y. (2022). The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes. Biofilm, 4, 100063. https://doi.org/10.1016/j.bioflm.2021.100063

Dong, G., Liu, H., Yu, X., Zhang, X., Lu, H., Zhou, T., & Cao, J. (2018). Antimicrobial and anti-biofilm activity of tannic acid against Staphylococcus aureus. Natural Product Research, 32(18), 2225–2228. https://doi.org/10.1080/14786419.2017.1366485

Fardiyah, Q., Suprapto, Kurniawan, F., Ersam, T., Slamet, A., & Suyanta. (2020). Preliminary Phytochemical Screening and Fluorescence Characterization of Several Medicinal Plants Extract from East Java Indonesia. IOP Conference Series: Materials Science and Engineering, 833(1), 012008. https://doi.org/10.1088/1757-899X/833/1/012008

Feuillolay, C., Pecastaings, S., Gac, C. L., Fiorini-Puybaret, C., Luc, J., Joulia, P., & Roques, C. (2016). A Myrtus communis extract enriched in myrtucummulones and ursolic acid reduces resistance of Propionibacterium acnes biofilms to antibiotics used in acne vulgaris. Phytomedicine, 23(3), 307–315. https://doi.org/10.1016/j.phymed.2015.11.016

Fissy, S. O. F., Sari, R., & Pratiwi, L. (2014). Efektivitas Gel Anti Jerawat Ekstrak Etanol Rimpang Jahe Merah (Zingiber officinale Rosc. Var. Rubrum) terhadap Propionibacterium acnes dan Staphylococcus epidermidis. Jurnal Ilmu Kefarmasian Indonesia, 12(2), 193–201. http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/148

Górniak, I., Bartoszewski, R., & Króliczewski, J. (2019). Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews, 18(1), 241–272. https://doi.org/10.1007/s11101-018-9591-z

Guzzo, F., Scognamiglio, M., Fiorentino, A., Buommino, E., & D’Abrosca, B. (2020). Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms. Molecules, 25(21), 5024. https://doi.org/10.3390/molecules25215024

Hong, W., Zeng, J., & Xie, J. (2014). Antibiotic drugs targeting bacterial RNAs. Acta Pharmaceutica Sinica B, 4(4), 258–265. https://doi.org/10.1016/j.apsb.2014.06.012

Jahns, A. C., & Alexeyev, O. A. (2014.). Three dimensional distribution of Propionibacterium acnes biofilms in human skin—Jahns—2014—Experimental Dermatology—Wiley Online Library. Retrieved December 31, 2023, from https://onlinelibrary.wiley.com/doi/10.1111/exd.12482

Kaczmarek, B. (2020). Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials—A Minireview. Materials, 13(14), 3224. https://doi.org/10.3390/ma13143224

Kementerian Kesehatan RI. (2017). Farmakope Herbal Indonesia Edisi II. Kementerian Kesehatan Republik Indonesia.

Kim, Y.-G., Lee, J.-H., & Lee, J. (2021). Antibiofilm activities of fatty acids including myristoleic acid against Cutibacterium acnes via reduced cell hydrophobicity. Phytomedicine, 91, 153710. https://doi.org/10.1016/j.phymed.2021.153710

Kusuma, I. W., Rahmini, Arung, E. T., Pramono, A. Y., Erwin, & Supomo. (2020). Biological activities and phytochemicals of Hyptis capitata grown in East Kalimantan, Indonesia. Journal of Applied Biology & Biotechnology, 8(2), 58–64. https://doi.org/10.7324/JABB.2020.80210

Lahiri, D., Dash, S., Dutta, R., & Nag, M. (2019). Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants. Journal of Biosciences, 44(2), 52. https://doi.org/10.1007/s12038-019-9868-4

Lim, V., Yap, C. S., Chong, H. W., Shukkoor, M. S. A., & Priya, M. (2015). Antimicrobial Evaluation and GC-MS Analysis of Strobilanthes crispus Ethanolic Leaf Extract. European Journal of Medicinal Plants, 10(4), 1–8. https://doi.org/DOI: 10.9734/EJMP/2015/20075

Linfante, A., Allawh, R. M., & Allen, H. B. (2017). The Role of Propionibacterium acnes Biofilm in Acne Vulgaris. Journal of Clinical & Experimental Dermatology Research, 09(01). https://doi.org/10.4172/2155-9554.1000439

Luchian, I., Goriuc, A., Martu, M. A., & Covasa, M. (2021). Clindamycin as an Alternative Option in Optimizing Periodontal Therapy. Antibiotics, 10(7), 814. https://doi.org/10.3390/antibiotics10070814

Mustarichie, R., Sulistyaningsih, S., & Runadi, D. (2020). Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne. International Journal of Microbiology, 2020, e1975904. https://doi.org/10.1155/2020/1975904

Ng, M. G., Ng, C. H., Ng, K. Y., Chye, S. M., Ling, A. P. K., & Koh, R. Y. (2021). Anticancer Properties of Strobilanthes crispus: A Review. Processes, 9(8), 1370. https://doi.org/10.3390/pr9081370

Samsulkahar, N. F., Hadi, A. A., Shamsuddin, M., & Malek, N. A. N. N. (2023). Biosynthesis of Gold Nanoparticles Using Strobilanthes crispa Aqueous Leaves Extract and Evaluation of Its Antibacterial Activity. Biointerface Research in Apllied Chemistry, 13(1), 1–16. https://doi.org/10.33263/briac131.063

Sangani, M. H., Moghaddam, M. N., & Mahdi, M. (2015). Inhibitory effect of zinc oxide nanoparticles on Pseudomonas aeruginosa. Nano Medicine Journal 2(2): 121-128. https://nmj.mums.ac.ir/article_4091_f60dfe6f0686f44e38fe15650fae8754.pdf

Stefan, R., Hobden, J. A., Miller, S., Morse, S. A., Mietzner, T. A., Detrick, B., Mitchell, T. G., Sakarani, J. A., Hotez, P., & Mejia, R. (2019). Jawetz, Melnick & Adelbreg’s: Medical Microbiology (28th ed.). McGraw Hill.

Suproborini, A., Laksana, M. S. D., & Martiningsih, S. H. (2022). Uji Efektivitas Antibakteri Ekstrak Daun Stobilanthes crispus Terhadap Pseudomonas aeruginosa. Journal of Pharmaceutical Science and Medical Research, 5(1), 25–32. DOI: http://doi.org/10.25273/pharmed.v5i1.12460.

Suproborini, A., Laksana, M. S. D., & Puspita, W. (2023). Strobilanthes crispus antibacterial against Aggregatibacter actinomycetecomitans. Asian Journal of Management, Entrepreneurship and Social Science, 3(03), 1150 – 1160. http://www.ajmesc.com/index.php/ajmesc/article/view/517/312

Taleb, M. H., Abdeltawab, N. F., Shamma, R. N., Abdelgayed, S. S., Mohamed, S. S., Farag, M. A., & Ramadan, M. A. (2018). Origanum vulgare L. Essential Oil as a Potential Anti-Acne Topical Nanoemulsion—In Vitro and In Vivo Study. Molecules, 23(9), 2164. https://doi.org/10.3390/molecules23092164

Wardani, H. N. (2020). Potensi Ekstrak Daun Sirsak Dalam Mengatasi Kulit Wajah Berjerawat. Jurnal Penelitian Perawat Profesional, 2(4), 563–570. DOI: https://doi.org/10.37287/jppp.v2i4.218

Yurlina Zai, Agnes Yohana Kristino, Sri Lestari Ramadhani Nasution, & Oliviti Natali. (2019). Uji Efektivitas Antibakteri Ekstrak Daun Sirsak (Annona muricata Linn,) Terhadap Bakteri Propionibacterium acnes. Jurnal Biologi Lingkungan, Industri, Kesehatan, 6(1), 59–64. https://doi.org/10.31289/biolink.v6i1.2244

DOI: https://doi.org/10.20961/jpscr.v9i1.74341

Refbacks

  • There are currently no refbacks.