The Potency of Moringa oleifera Leaves Extract as Larvicide for Aedes aegypti

Kusmiyati Kusmiyati, Ety Rahmawati, Edwin Mesach Mauguru, Ferry William Frangky Waangsir, Pius Selasa

Abstract

Abstract

Background: The main vector of dengue fever is the Aedes aegypti. The use of synthetic insecticides, especially larvicides, has several effects, including resistance and environmental pollution. Therefore, researchers are interested in examining the potential of Moringa plants as natural larvicides.  This study was conducted for knowing influence administration of M. oleifera leaf extract against the death of Aedes aegypti  larvae and effective concentration  as a larvicide against Aedes aegypti larvae.

Method: This research was a true experiment, using design randomized post test only control group design.  The research test was conducted by dividing the sample into 6 groups, where 1 group was the control and 5 groups were treated with M. oleifera leaf extract, consentrations of 100 ppm, 200 ppm, 300 ppm, 400 ppm and 500 ppm.

Results:  The results of the Kruskal-Wallis statistical test on the effect of M. oleifera leaf extract on larval mortality during 24 hours of exposure showed a significant effect (p value 0.002), while at 48 hours exposure it also showed the same result (p = 0.004). This result showed that there was a significant difference between control group and treatment groups at concentration 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm (p value 0.000).

Conclusion: There is an effect of giving M. oleifera leaf extract on the mortality of Aedes aegypti larvae. Various concentrations were effective in causing the death of Aedes aegypti mosquito larvae . It is needed to observe the mortality of Aedes aegypti larvae at shorter observation intervals and varying extract concentrations.

 

Keywords

Aedes aegypti; larvacide; M. oleifera

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References

Nadjib M, Ery S, Septiara P, Joshua N, Sophie B, Rezeki H, et al. Economic burden of dengue in Indonesia. PLoS Negl Trop Dis. 2019;Januari(10):1–14. 2. Murugesan A, Manoharan M. Dengue virus [Internet]. Emerging and Reemerging Viral Pathogens: Volume 1: Fundamental and Basic Virology Aspects of Human, Animal and Plant Pathogens. Elsevier Inc.; 2019. 281–359 p. Available from: http://dx.doi.org/10.1016/B978-0-12-819400-3.00016-8 3. Egid BR, Coulibaly M, Dadzie SK, Kamgang B, McCall PJ, Sedda L, et al. Review of the ecology and behaviour of Aedes aegypti and Aedes albopictus in Western Africa and implications for vector control. Curr Res Parasitol Vector-Borne Dis. 2022;2(December 2021):100074. 4. Faustino CG, de Medeiros FA, Ribeiro Galardo AK, Lobato Rodrigues AB, Lopes Martins R, de Medeiros Souza Lima Y, et al. Larvicide Activity on Aedes aegypti of Essential Oil Nanoemulsion from the Protium heptaphyllum Resin. Molecules. 2020;25(22). 5. Rampadarath S, Puchooa D, Ranghoo-Sanmukhiya VM. Antimicrobial, phytochemical and larvicidal properties of Jatropha multifida Linn. Asian Pac J Trop Med. 2014;7(S1):S380–3. 6. Ishak AR, Dom NC, Hussain H, Sabri NH. Biolarvacidal Potential of Ipomoea Cairica Extracts Against Key Dengue Vectors. Procedia - Soc Behav Sci [Internet]. 2014;153:180–8. Available from: http://dx.doi.org/10.1016/j.sbspro.2014.10.052 7. Kou X, Li B, Olayanju JB, Drake JM, Chen N. Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients. 2018;10(3). 8. Valdez-Solana MA, Mejía-García VY, Téllez-Valencia A, García-Arenas G, Salas-Pacheco J, Alba-Romero JJ, et al. Nutritional content and elemental and phytochemical analyses of moringa oleifera grown in Mexico. J Chem. 2015;2015. 9. Al-Barty A, Hamza R. Larvacidal, Antioxidant Activities and Perturbation of Transminases Activities of Titanium Dioxide Nanoparticles Synthesized Using Moringa oleifera Leaves Extract Against The Red Palm Weevil (Rhynchophorus ferrugineus). Eur J Pharm Med Res. 2015;2(6):49–54. 10. Kumar S, Astalakshmi N, Anagha C., Aparna P, Lulushad N, Vijisha C., et al. Evaluation of Moringa oleifera Lam Leaves, Flowers and Fruits Aqueous Extract For Larvacidal Property. World J Pharm Pharm Sci. 2016;Vol 5(4):1892–6. 11. Chinenyenwa O, Godson A. Bio-insecticidal efficacy of Moringa oleifera on the malaria vector, Anopheles and toxicity evaluation on fish behaviour. Int J Mosq Res. 2017;4(2):85–92. 12. Silva LL de S, Santos ND de L, Silva SCC, Lima T de A, Coelho LCBB, Zingali RB, et al. Investigation of the ability of the oviposition-stimulant lectin from Moringa oleifera seeds (WSMoL) to bind with membrane proteins present in the legs of Aedes aegypti. Int J Biol Macromol [Internet]. 2020;162:657–62. Available from: https://doi.org/10.1016/j.ijbiomac.2020.06.189 13. Ndii MZ. The effects of vaccination, vector controls and media on dengue transmission dynamics with a seasonally varying mosquito population. Results Phys [Internet]. 2022;34:105298. Available from: https://doi.org/10.1016/j.rinp.2022.105298 14. Hamid PH, Ninditya VI, Prastowo J, Haryanto A, Taubert A, Hermosilla C. Current Status of Aedes aegypti Insecticide Resistance Development from Banjarmasin, Kalimantan, Indonesia. Biomed Res Int. 2018;2018. 15. Hamid P., Prastowo J, Ghiffari A, Taubert A, Hermosilla C. Aedes aegypti resistance development to commonly used insecticides in Jakarta, Indonesia. PLoS One. 2017;12(12):1–11. 16. de Oliveira APS, de Santana Silva LL, de Albuquerque Lima T, Pontual EV, de Lima Santos ND, Breitenbach Barroso Coelho LC, et al. Biotechnological value of Moringa oleifera seed cake as source of insecticidal lectin against Aedes aegypti. Process Biochem [Internet]. 2016;51(10):1683–90. Available from: http://dx.doi.org/10.1016/j.procbio.2016.06.026 17. Sharma E, Mathur P, Kumar D, Srivastava M, Prasad A. Larvicidal Activity of Moringa oleifera Leaves against Human Malaria Vector Anopheles stephensi Liston(Insecta: Diptera: Culicidae). : In: XII International Conference onVector and Vector Borne Diseases—Challenges in 21st Century: Their Global Impact and Strategic Management. 2013. p. 117–22. 18. Rohmah EA, Subekti S, Rudyanto M. Larvicidal Activity and Histopathological Effect of Averrhoa bilimbi Fruit Extract on Aedes aegypti from Surabaya, Indonesia. J Parasitol Res. 2020;2020. 19. Ilham R, Lelo A, Harahap U, Widyawati T, Siahaan L. The effectivity of ethanolic extract from papaya leaves (Carica papaya L.) as an alternative larvacide to Aedes spp. Open Access Maced J Med Sci. 2019;7(20):3395–9. 20. Prasad A, Sharma E. Phytotoxicological Assesment of Moringa oleifera Lam. Against Larvae of Important Human Malaria Vector Anopheles stephensi Liston (Insecta: Diptera: Culicidae). Int J Innov Appl Stud. 2014;7(4):1633–41. 21. Ejeta D, Asme A, Asefa A. Insecticidal effect of ethnobotanical plant extracts against Anopheles arabiensis under laboratory conditions. Malar J [Internet]. 2021;20(1):1–8. Available from: https://doi.org/10.1186/s12936-021-04004-6 22. Saini RK, Sivanesan I, Keum YS. Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech. 2016;6(2):1–14. 23. Yuliasih Y, Widawati M. Larvicidal Activity of Various Solvents of Pongamia pinnata Seed E xtract on the Mortality of Aedes s pp . BALABA. 2017;13(2):125–32. 24. Silva LL de SLS, Silva SCCCC, de Oliveira APSPS, Nascimento J da SS, Silva E de OO, Coelho LCBB, et al. Effects of a solid formulation containing lectin-rich fraction of Moringa oleifera seeds on egg hatching and development of Aedes aegypti larvae. Acta Trop. 2021;214(November 2020). 25. Yasi R, Harsanti R. The Larvacidal Activity of Moringa aloifera Extract Leaf to The Larva’s Aedes aegypti Mortality. J Agromedicine Med Sci. 2018;4(3):12–7.

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