Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses

Sri Hartati, Ahmad Yunus, Iistikharoh Iistikharoh

Abstract


The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between Coelogyne pandhurata and Coelogyne rumphii. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.


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References


  1. Rezaei A, Hosseipour B. In vitro propagation and rooting of garnem rootstock. Plant Cell Biotech Mol Bio. 2015; 16(1-2): 41 – 47.
  2. Feng J, Shi Y, Yang S, Zuo J. Hormone metabolism and plant signaling. Academic Press. 2017; 77-106.
  3. Mutasim M, Eisa K, Elgaali I. In vitro multiple shoot regeneration from nodal explants of Vernonia amygdalana, an important medicinal plant. Af Crop Science. 2017; 8(2): 747–752.
  4. Nurana AR, Wijana G, Dwiyani R. Effect of 2-ip and NAA on plantlet growth of hybrid dendrobium orchids at the subculture stage. Agrotrop. 2017; 7(2): 139–146.
  5. Ni Made, Hartini M, Rauf G. In Vitro Proliferation of Plb Orchid Dendrobium lasianthera Result of Genetic Mutation Induction with Colchicine Through Addition of Benzyl Adenine. Bul Agrohorti. 2017; 5(2): 146-156.
  6. Kumari N, Pragiti M. Mass in vitro micropropagation of banana Musa sp. International J of Plant Protection. 2016; 9(1): 204–210.
  7. Indriani BS, Enni S, Krispinus. Efektivitas substitusi sitokinin dengan air kelapa pada multiplikasi tunas krisan secara in vitro. J Life Science. 2017; 3(2): 148–155.
  8. Zahara M, Avishek D, Patchareeya B. Effect of plant growth regulators on the growth and direct shoot formation from leaf explants of the hybrid phalaenopsis ‘pink.’ Acta Agriculturae Slovenica. 2018; 111(1): 5–16.
  9. Kartiman R, Dewi S, Syarifah IA. Multiplikasi in vitro anggrek hitam (Coelogyne pandurata lindl.) pada perlakuan kombinasi NAA dan BAP. J Bioteknologi dan Biosains Indonesia. 2018; 5(1): 75–87.
  10. Garvita ES. Effect of 6-benzyl amino purine on the structure of photosynthetic apparatus of faba bean (Vicia faba L.). Appl Biochem Microbiol. 2019; 39: 411–417.
  11. Jitsopakula N, Kachit T, Keiko L. Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid. Science Asia. 2013; 39(3): 449–455.
  12. Bohidar S, Thirunavoukkarasu M, Roa TV. Effect of plant growth regulators on in vitro micropropagation of ‘garden rue’ (R. graveolens L.). International Journal of Integrated Biology. 2018; 3(1): 36-43.
  13. Kriswanto B, Sigit S, Didik PR. The propagation of orchid through formation of protocorm like bodies (plb) for supporting the resque of phalaenopsis orchid in Indonesia. Proceedings of the international conference of Fossa. J Biosfera. 2017; 34(1): 9–14.
  14. Hajare ST, Nitin MC, Girum C. Effect of Growth Regulators on In Vitro Micropropagation of Potato (Solanum tuberosum L.) Gudiene and Belete Varieties from Ethiopia. The Scientific Word Journal. 2021; 1(1): 1–8.
  15. Ngomuo M, Emerald M, Patrick N. The effects of auxins and cytokinin on growth and development of (Musa sp.) Var. “Yangambi” Explants in Tissue Culture. American Journal of Plant Sciences. 2018; 4(2): 2174-2180.
  16. Maharjan S, Pradhan S, Thapa BB, Pant B. In Vitro Propagation of Endangered Orchid, Vanda pumila Hook.f. through Protocorms Culture. American Journal of Plant Sciences. 2019; 10(7): 1220-1232.
  17. Pradhan S, Tiruwa BL, Subedee BR. Efficient plant regenration of Cympidium aloifolium, a threaened orchid of nepal through artificial seed technology. American J of Plant Science. 2017; 7(2): 1964–1974.
  18. Ansari ZNE, Amina EM, Ihtissam B. Effect of macronutrients, cytokinins, and auxins on in vitro organogenesis of thymus vulgaris L. American Journal of Plant Sciences. 2019; 10(9): 1482-1502.
  19. Siron U, Noertjahyani, Yana T, Romiyadi. Pengaruh konsentrasi zat pengatur tumbuh naphthalene acetic acid dan benzil amino purin terhadap pertumbuhan protokorm anggrek Dendrobium spectabile pada kultur in vitro. Paspalum: Jurnal Ilmiah Pertanian. 2019; 7(1): 16–23.
  20. Shoemaker RC, Amberger LA, Palmer RG. Effect 2.4-dichlorophenoxy acetic acid concentration on somatic embryogenesis and heretable variation in soybean (Glycine max (L.) Merr)’, In vitro Cell Dev. Biology. 2015; 27(2): 84-89.
  21. Shah ZM, Quraishi A, Hassan G. Meristem culture of potato (Solanum tuberosum L.) for producing virus-free plantlets. Journal of Biological Sciences. 2018; 1(2): 898-899.
  22. Pather C. Effect of naphtaleine acetic acid (NAA) on somatic embryogenesis of the orchid Phalaenopsis amabilis (L.). Biodiversitas. 2017; 5(1&2): 30-35.
  23. Goswami K, Yasmin S, Nasruddin KM. In Vitro Regeneration of Dendrobium sp. of Orchid Using Leaf Tip as Explant. J Environ Sci Natural Resources. 2015; 8(2): 75–78.
  24. Hussein N. Effects of nutrient media constituent on growth and development of banana (Musa sp.) shoot tips cultures in vitro. Africal J of Biotechnology, 2021; 11(37): 233–239.




DOI: https://doi.org/10.20961/jbb.v3i2.80626

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