Risk Factors for H9N2 Contamination in Live Bird Markets of the Greater Jakarta, Indonesia

Rahma Hani Aisah, Bambang Sumiarto, Hendra Wibawa, Widagdo Sri Nugroho, Aprizal Panus, Sodirun Sodirun

Abstract


Low Pathogenicity Avian Influenza virus subtype H9N2 causes decreased egg production and two of the four defined H9N2 poultry lineages (G1 and Y280) have been associated with human infections. The high positive test results for the H9N2 virus in the LBMs of the Greater Jakarta area in 2021 can be a source of transmission for the surrounding area. This study aims to determine the prevalence and risk factors that play a role in the H9N2 contamination in the LBMs to obtain a multivariate analysis model. A cross-sectional study was conducted on 87 vendors and 124 broiler chickens from 44 LBMs randomly selected in Tangerang City, North Jakarta, and Bekasi City. Samples were taken as equipment swabs at the stall of poultry carcass vendors, tracheal swabs, and cloacal swabs of broiler chicken. The test used was Real-Time Reverse Transcription Polymerase Chain Reaction. Data analysis was performed using univariate, bivariate, and multivariate analysis with logistic regression. The prevalence of H9N2 virus contamination at the LBM level in Greater Jakarta in 2022 was 77.27% (95% CI, α 7%), while the prevalence at the vendor level was 54.02% (95% CI, α 5%). The risk factors for H9N2 virus contamination with a significant p-value<0.05 include the positive results on equipment swab samples, positive results on both equipment and broiler swabs, and the use of carcass sink.  The multivariate analysis model of H9N2 virus contamination at the vendor level was Logit P (H9N2=1 | x) = 0.29924 - 2.42176PPE + 1.58691carcass sink. The risk factor contributing to increase H9N2 contamination is using carcass sinks, while PPE is the one that reduces H9N2 contamination.

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References


  1. Liu, D., Shi, W., Shi, Y., et al. Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses. Lancet. 2013; 381: 1926-1932
  2. Chen, H., Yuan, H., Gao, R., et al. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet. 2014; (published online Feb 5.) https://doi.org/10.1016/S0140-6736(14)60111-2
  3. Shen, Y.Y.; Ke, C.W.; Li, Q.; Yuan, R.Y.; Xiang, D.; Jia, W.X.; Yu, Y.D.; Liu, L.; Huang, C.; Qi, W.B.; et al. Novel reassortant avian influenza A(H5N6) viruses in humans, Guangdong, China, 2015. Emerg. Infect. Dis. 2016; 22, 1
  4. Peacock, T.P., James, J., Sealy, J.E., Iqbal, M. A. Global Perspective on H9N2 Avian Influenza Virus. Viruses 2019; 11, 620. https://doi.org/10.3390/v11070620
  5. Fournié, G., J. Guitian, S. Desvaux, V.C. Cuong, D. Huu Dung, D.U. Pfeiffer, P. Mangtani, A.C. Ghani. 2013. Interventions for avian influenza A (H5N1) risk management in live bird market networks. Proc. Natl. Acad. Sci. 2013;110 (22), pp. 8751-8752
  6. Thuy DM, Peacock TP, Bich VTN, Fabrizio T, Hoang DN, Tho ND, Diep NT, Nguyen M, Hoa LNM, Trang HTT, Choisy M, Inui K, Newman S, Trung NV, van Doorn R, To TL, Iqbal M, Bryant JE. Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infect Genet Evol. 2016 Oct;44:530-540. doi: 10.1016/j.meegid.2016.06.038. Epub 2016 Jun 20. PMID: 27340015; PMCID: PMC5036934.
  7. Chaudhry M, et al. Avian influenza A in poultry stalls of Lahore District, Pakistan in 2009-2010. J Infect. 2017;74:609–611. doi: 10.1016/j.jinf.2017.03.008.
  8. Pepin KM, Wang J, Webb CT, Smith GJ, Poss M, Hudson PJ, et al. Multiannual patterns of influenza A transmission in Chinese live bird market systems. Influenza Other Respi Viruses. 2013; 7(1):97–107.
  9. Chen LJ, Lin XD, Tian JH, Liao Y, Ying XH, Shao JW, et al. Diversity, evolution and population dynamics of avian influenza viruses circulating in the live poultry markets in China. Virology. 2017;505:33–41.
  10. Dharmayanti NLPI, Hewajuli DA, Ratnawati A, Hartawan R. Genetic diversity of the H5N1 viruses in live bird markets, Indonesia. J Vet Sci. 2020 Jul;21(4):e56. doi: 10.4142/jvs.2020.21.e56. PMID: 32735094; PMCID: PMC7402941.
  11. Indriani, R., G. Samaan, A. Gultom, L. Loth, S. Indryani, R. Adjid, N.L. Putu Indi Dharmayanti, J. Weaver, E. Mumford, K. Lokuge, P.M. Kelly, Darminto. Environmental sampling for avian influenza virus A (H5N1) in live-bird markets, Indonesia. Emerg. Infect. Dis. 2010; 16, pp. 1889-1895
  12. Yu H, Wu JT, Cowling BJ, Liao Q, Fang VJ, Zhou S, et al. Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study. Lancet. 2014;383(9916):541–548.
  13. Jonas, M., Sahesti, A., Murwijati, T., Lestariningsih, C. L., Irine, I., Ayesda, C. S., … Mahardika, G. N. Identification of avian influenza virus subtype H9N2 in chicken farms in Indonesia. Preventive Veterinary Medicine 2018; 159, 99–105. doi:10.1016/j.prevetmed.2018.09.003
  14. Lestari, H. Wibawa, E. P. Lubis, R. Dharmawan, R. A. Rahayu, H. Mulyawan, K. Charoenkul, C. Nasamran, B. Poermadjaja, & A. Amonsin. Co-circulation and characterisation of HPAI-H5N1 and LPAI-H9N2 recovered from a duck farm, Yogyakarta, Indonesia. Transbound Emerg. Dis. 2019; 67:994–1007. https://doi.org/10.1111/tbed.13434
  15. Muflihanah, E. Andesfha, H. Wibawa, F. C. Zenal, F. Hendrawati, Siswani, Wahyuni, D. Kartini, I. Rahayuningtyas, S. Hadi, S. Mukartini, B. Poermadjaja, & F. S. T. Rasa. Kasus pertama low pathogenic Avian Influenza Subtipe H9N2 pada peternakan ayam petelur di Kabupaten Sidrap, Sulawesi Selatan Indonesia. Buletin InfoVet 2017;16:1-13
  16. Martin, S.W., Meek, A.H. and Wilberg, P. Veterinary Epidemiology: Principles and Methods. Iowa State University Press, Ames; 1988.
  17. Direktorat Jenderal Peternakan dan Kesehatan Hewan Kementerian Pertanian. Prosedur Operasional Baku Deteksi Virus Avian Influenza dengan Teknik Real-Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR); 2018.
  18. Smith, R.D. Veterinary Clinical Epidemiology From Patient to Population Fourth Edition. New York: CRC Press Taylor & Francis Group; 2020.
  19. Bertran, K., C. Balzli, Y.K. Kwon, T.M. Tumpey, A. Clark, and D.E. Swayne. Airborne transmission of highly pathogenic influenza virus during processing of infected poultry. Emerg Infect Dis. 2017;23:1806–1814.
  20. Henning J, Hesterberg UW, Zenal F, Schoonman L, Brum E, McGrane J. Risk factors for H5 avian influenza virus prevalence on urban live bird markets in Jakarta, Indonesia—Evaluation of long-term environmental surveillance data. PLoS ONE 2019; 14(5): e0216984. https://doi.org/10.1371/journal.pone.0216984




DOI: https://doi.org/10.20961/jbb.v3i1.74263

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