1.png){kind=logo}
3.png)
In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations
Abstract
Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time.
Keywords: explant; plant growth; tissue culture.
Full Text:
PDFReferences
Sharifi S. Enhanced callus induction and high-efficiency plant regeneration in Tribulus terrestris L., an important medicinal plant. Journal of Medicinal Plants Research, 2012;6(27):4401-4408. https://doi.org/10.5897/jmpr12.260.
Austin DF.. Handbook of medicinal herbs. Second Edition. In Economic Botany, 2006;57(2):290.https://doi.org/10.1663/0013-0001(2003)057[0290:homhse]2.0.co;2
Xiong W, Luo G, Zhou L, Zeng Y, Yang W. In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures. Chinese Medicine, 2009;4:1-7. https://doi.org/10.1186/1749-8546-4-14
Jiji MJ, Visalakshi S, Meenakshi P, Rathi MA, Thirumoorthi L, Chinna GD, Gopalakrishnan VK. Antilipidemic activity of Cissus quadrangularis and Tribulus terrestris on obesity in high fat fed rats. Pharmacologyonline, 2009;2: 1250-1258.
Raghu AV, Geetha SP, Martin G, Balachandran I, Mohanan KV. Micropropagation of Tribulus terrestris Linn. Indian Journal of Natural Products and Resources, 2010;1(2): 232-235.
Armas I, Pogrebnyak N, Raskin I. A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.). Plant Methods, 2017;13(1):1-9.
Pinhal HF, Araruna E da C, Carneiro PAP, Asmar SA, Melo B de, Luz JMQ. Concentration of MS medium and cutting of seeds on in vitro establishment of baruzeiro (Dipteryx alata Vog.). Bioscience Journal, 2017;33(2):306-313.
Valdez M, Gatica-AAM. Effect of BAP and IAA on shoot regeneration in cotyledonary explants of Costa Rican melon genotypes. Agronomia Costarricense, 2009;33(1): 125-131.
Aloni R, Schwalm K, Langhans M, Ullrich CI. Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis. Planta, 2003:(5):841-853.
Gravel V, Antoun H, Tweddell RJ. Effect of indole-acetic acid (IAA) on the development of symptoms caused by Pythium ultimum on tomato plants. European Journal of Plant Pathology, 2007;119(4):457-462.
Peixe A, Raposo A, Lourenço R, Cardoso H, Macedo E. Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation. Scientia Horticulturae, 2007;113(1): 1-7.
Antony CS, Lenin MS, Bhargav PK, Karthigan M, Ignacimuthu S. Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiologiae Plantarum, 2010;32(3): 443-452.
Mazri MA. Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm (Phoenix dactylifera L.) cv. Boufeggous. Journal of Plant Biochemistry and Biotechnology, 2015;24(3): 268-275.
Mathieu RA, Cournède PH, de Reffye P. A dynamical model of plant growth with full retroaction between organogenesis and photosynthesis. Arima, 2006;4: 101-107.
Juvany M, Müller M, Munné-Bosch S. Plant age-related changes in cytokinins, leaf growth and pigment accumulation in juvenile mastic trees. Environmental and Experimental Botany, 2013;87:10-18. https://doi.org/10.1016/j.envexpbot.2012.09.007
Nakhooda M, Watt MP, & Mycock D. Auxin stability and accumulation during in vitro shoot morphogenesis influences subsequent root induction and development in Eucalyptus grandis. Plant Growth Regulation, 2011;65(2): 263-271. https://doi.org/10.1007/s10725-011-9597-7
Vanneste S, Friml J. Auxin: a trigger for change in plant development. Cell, 2009;136(6): 1005-1016.
Kim HJ, Ryu H, Hong SH, Woo HR, Lim, PO, Lee IC., Hwang I. Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. Proceedings of the National Academy of Sciences, 2006;103(3): 814-819.
Kuderová A, Urbánková I, Válková M, Malbeck J, Brzobohatý B, Némethová D, & Hejátko J. Effects of conditional IPT-dependent cytokinin overproduction on root architecture of arabidopsis seedlings. Plant and Cell Physiology, 2008;49(4): 570-582.
Kurakawa T, Ueda N, Maekawa M, Kobayashi K, Kojima M, Nagato Y, Kyozuka, J. Direct control of shoot meristem activity by a cytokinin-activating enzyme. Nature, 2007;445(7128): 652-655. https://doi.org/10.1038/nature05504
Riefler M, Novak O, Strnad M, Schmülling T. Hospital uses EMR to improve handoff process and create electronic “Hall Pass". The Plant Cell, 2006;18: 40-54.
Tran LP, Urao T, Qin F, Maruyama K, Kakimoto T, Shinozaki K, Yamaguchi-shinozaki K.. Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis. PNAS, 2007;104(51): 3-8. https://doi.org/10.1073/pnas.0706547105
Hamad AM, Taha RM. Effect of benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas comosus L. Merr.) cv. smooth cayenne. Journal of Applied Sciences, 2008;8(22): 4180-4185.
Zhang JY, Guo ZR, Zhang R, Li YR, Cao L, Liang YW, Huang LBin. Auxin type, auxin concentration, and air and substrate temperature difference play key: Roles in the rooting of juvenile hardwood pecan cuttings. HortTechnology, 2015; 25(2): 209-213.
Leyser O. Dynamic integration of auxin transport and signalling. Current Biology, 2006; 16(11): 424-433.
Reinhardt D, Mandel T, & Kuhlemeier C. Auxin regulates the initiation and radial position of plant lateral organs. The Plant Cell, 2007; 12(4): 507-518.
Sassi M, Vernoux T. Auxin and self-organization at the shoot apical meristem. Journal of Experimental Botany, 2013;64(9): 2579-2592.
Karcz W, Burdach Z. Effect of temperature on growth, proton extrusion and membrane potential in maize (Zea mays L.) coleoptile segments. Plant Growth Regulation, 2007;52(2): 141-150. https://doi.org/10.1007/s10725-007-9184-0
Arimarsetiowati R, Ardiyani F. Pengaruh penambahan auxin terhadap pertunasan dan perakaran kopi arabika perbanyakan Somatik Embriogenesis. Pelita Perkebunan, 2012:28(2):82-90.
Akhiriana E, Samanhudi, Yunus A. Coconut water and Iaa effect on the in vitro growth of Tribulus terrestris L. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2019;67(1): 9-18. https://doi.org/10.11118/actaun201967010009
Kristina NNSFS. Pengaruh air kelapa muda terhadap multiplikasi tunas in vitro, produksi rimpang, dan kandungan xanthorrhizol temulawak di lapangan. Jurnal Litrri, 2012;18, 126-134.
Rahmawati F, Wicaksono KP, Aini N. Pengaruh fermentasi urin kelinci dan paitan terhadap pertumbuhan dan hasil tanaman paprika (Capsicum annuum var Grossum) dengan sistem hidroponik subtrate. Jurnal Produksi Tanaman, 2018;6(2), 194-202.
Prabowo H, Samanhudi, Yuniastuti E, Yunus A. Effects of media combination with concentration of ab-mix nutrient on growth of banana shoots on in vitro. Bulgarian Journal of Agricultural Science, 2018;24(3): 404-410.
DOI: https://doi.org/10.20961/jbb.v1i1.50245
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
|
