Comparative Cytogenetic Characterization of Grammatophyllum martae, Dendrobium violaceoflavens, and Dendrobium Tiara Beauty × D. Graty

Sri Hartati, Fitria Roviqowati, Yuni Tuwenti

Abstract


Orchids are among the most popular ornamental plants widely favored by the public. Plant breeders continuously strive to improve the quantity, quality, and cytological characteristics of orchids to support successful breeding programs. This study aimed to determine the cytological characteristics of Grammatophyllum martae, Dendrobium violaceoflavens, and the hybrid D. Tiara Beauty × D. Graty, including chromosome number, chromosome size, chromosome shape, karyotype, and chromosome asymmetry index. The research was conducted at the Plant Breeding Laboratory, Faculty of Agriculture, Universitas Sebelas Maret, from July to August 2025. The number, size, shape, and karyotype pattern of chromosomes were analyzed descriptively. The results showed that G. martae, D. violaceoflavens, and the hybrid D. Tiara Beauty × D. Graty have the same chromosome number, 2n = 38, composed of metacentric chromosomes. The chromosomes of G. martae had a total arm length of 1.10 ± 0.28 µm, D. violaceoflavens 1.48 ± 0.40 µm, and the hybrid D. Tiara Beauty × D. Graty 1.30 ± 0.36 µm. The intrachromosomal (A1) and interchromosomal (A2) asymmetry indices of the three observed orchids were low, indicating that their karyotypes tend to be symmetrical, as they are composed of metacentric chromosomes with minimal size variation.


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References


  1. Badan Pusat Statistik. Produksi tanaman florikultura (hias) 2023. Jakarta: Badan Pusat Statistik Republik Indonesia; 2023.
  2. Iiyama CM, Vilcherrez-Atoche JA, Germanà MA, et al. Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century. Heredity. 2024;132(4):163-178.
  3. Mayrose I, Lysak MA. The evolution of chromosome numbers: mechanistic models and experimental approaches. Genome Biol Evol. 2021;13(2):evab220.
  4. Atoche JA, Iiyama CM, Cardoso JC. Polyploidization in orchids: from cellular changes to breeding applications. Plants. 2022;11(4):469.
  5. Rodionov AV, Amosova AV, Belyakov EA, et al. Genetic consequences of interspecific hybridization, its role in speciation, and phenotypic diversity of plants. Russ J Genet. 2019;55(3):278-294.
  6. Ramesh T, Kumari KG, Muruganantham P, et al. Cytological studies on some endemic terrestrial orchids. Plant Arch. 2022;22(1):151-157.
  7. Chao YT, Chen WC, Chen CY, et al. Chromosome level assembly, genetic and physical mapping of the Phalaenopsis aphrodite genome provides new insights into species adaptation and resources for orchid breeding. Plant Biotechnol J. 2018;16(12):2027-2041.
  8. Hsu CC, Chen SY, Lai PH, et al. Identification of high-copy number long terminal repeat retrotransposons and their expansion in Phalaenopsis orchids. BMC Genomics. 2020;21:807.
  9. Mitrenina EY, Alekseeva SS, Badaeva ED, et al. Karyotypes and physical mapping of ribosomal DNA with oligo-probes in Eranthis sect. Eranthis (Ranunculaceae). Plants. 2024;13(1):47.
  10. Turco A, Wagensommer RP, Medagli P, et al. Advances in the study of Orchidinae subtribe (Orchidaceae) species with 40, 42-chromosomes in the Mediterranean region. Diversity. 2024;16(1):20.
  11. Hartati S, Cahyono O, Wibowo A, et al. The chromosome of Phalaenopsis spp. and Doritaenopsis sp. hybrid induced by colchicine. IOP Conf Ser Earth Environ Sci. 2023;1133(1):012030.
  12. Hartati S, Rifai K. Chromosome Dendrobium orchid natural. IOP Conf Ser Earth Environ Sci. 2025;1490:012052.
  13. Hartati S. Orchids cytogenetics and karyotype of Phaius callosus and Phaius montanus. J Ilm Agineca. 2022;22(2):26-32.
  14. Jain AK, Singh D, Dubey K, et al. Chromosomal aberrations. In: Mutagenicity: Assays and Applications. London: Academic Press; 2018. p. 69-92.
  15. Morrison O, Thakur J. Molecular complexes at euchromatin, heterochromatin, and centromeric chromatin. Int J Mol Sci. 2021;22(13):6922.
  16. Allshire RC, Madhani HD. Ten principles of heterochromatin formation and function. Nat Rev Mol Cell Biol. 2018;19(4):229-244.
  17. Vimala Y, Lavania S, Lavania UC. Chromosome change and karyotype differentiation: implications in speciation and plant systematics. Nucleus. 2021;64(1):33-54.
  18. Muliawati E, Hartati S, Parjanto, et al. Karyotype of Phaius tankervilleae and Phaius amboinensis orchid. E3S Web Conf. 2023;373:00001.
  19. Martin E, Yıldız HK, Kahraman A, Binzat OK, Eroğlu HE. Detailed chromosome measurements and karyotype asymmetry of some Vicia (Fabaceae) taxa from Turkey. Caryologia. 2018;71(3):224-232.
  20. Cornforth MN, Durante M. Radiation quality and intra-chromosomal aberrations: size matters. Mutat Res Genet Toxicol Environ Mutagen. 2018;836:28-35.




DOI: https://doi.org/10.20961/jbb.v6i1.117680

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