Pengaruh Pemberian Agen Kontras Pewarna Sintetik pada Jaringan Biologis terhadap Hasil Pencitraan Fotoakustik

Miftahul Janna, Mitrayana Mitrayana, Rini Widyaningrum

Abstract

An experiment to investigate the influence of synthetic dye contrast agent in photoacoustic imaging of biological tissue was conducted in this study. This study uses a simple phocoacoustic imaging system consists of three main components, i.e., a diode laser, condenser microphone, and a custom-build X-Y stage. Characterization was performed on the main components of the system to obtain the appropriate settings on imaging the biological tissue in this study. The results of the optimal frequency and duty cycle for laser modulation in this study were 19000 Hz and duty cyle of 40%, respectively. The addition of a contrast agent aims to improve the quality of the image by comparing the sample with methylene blue contrast agent, the sample with methyl red and the sample without contrast agent. The increases of acoustic intensity level is in proportion with the contrast agent concentration. The difference in the photoacoustic images reveal that the sample with methylene blue contrast agent has the highest acoustic intensity level compared to both sample with methyl red contrast agent and the sample without contrast agent. Therefore, this research proves that a photoacoustic imaging system can be developed to image biological tissue with a contrast agent and methylene blue has greater potential than methyl red to be used as a contrast agent in photoacoustic imaging.

Keywords

contrast agent, biological tissue, photoacoustic imaging, syntetic dye.

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References

  1. C. Pomara, N. Pascale, F. Maglietta, M. Neri, I. Riezzo, and E. Turillazzi, “Use of contrast media in diagnostic imaging: medico-legal considerations,” Radiol. Medica, vol. 120, no. 9, pp. 802–809, 2015, doi: 10.1007/s11547-015-0549-6.
  2. Q. Yao, Y. Ding, G. Liu, and L. Zeng, “Low-cost photoacoustic imaging systems based on laser diode and light-emitting diode excitation,” J. Innov. Opt. Health Sci., vol. 10, no. 4, pp. 1–13, 2017, doi: 10.1142/S1793545817300038.
  3. W. W. Liu and P. C. Li, “Photoacoustic imaging of cells in a three-dimensional microenvironment,” J. Biomed. Sci., vol. 27, no. 1, pp. 1–9, 2020, doi: 10.1186/s12929-019-0594-x.
  4. Y. Zhang, H. Hong, and W. Cai, “Photoacoustic imaging,” Cold Spring Harb. Protoc., vol. 6, no. 9, pp. 1015–1025, 2011, doi: 10.1101/pdb.top065508.
  5. Y. Wu, H. K. Zhang, J. Kang, and E. M. Boctor, “An economic photoacoustic imaging platform using automatic laser synchronization and inverse beamforming,” Ultrasonics, vol. 103, p. 106098, 2020, doi: 10.1016/j.ultras.2020.106098.
  6. D. Xu, S. Yang, Y. Wang, Y. Gu, and D. Xing, “Noninvasive and high-resolving photoacoustic dermoscopy of human skin,” Biomed. Opt. Express, vol. 7, no. 6, p. 2095, 2016, doi: 10.1364/boe.7.002095.
  7. U. Dahlstrand et al., “Photoacoustic imaging for three-dimensional visualization and delineation of basal cell carcinoma in patients,” Photoacoustics, vol. 18, no. March 2019, pp. 1–7, 2020, doi: 10.1016/j.pacs.2020.100187.
  8. Y. Lin, Z. Li, Z. Li, J. Cai, H. Wui, and H. Li, “Real-time photoacoustic and ultrasonic dual-modality imaging system for early gastric cancer: Phantom and ex vivo studies,” Opt. Commun., vol. 426, no. November 2017, pp. 519–525, 2018, doi: 10.1016/j.optcom.2018.05.087.
  9. C. Moore et al., “Photoacoustic imaging for monitoring periodontal health: A first human study,” Photoacoustics, vol. 12, no. August, pp. 67–74, 2018, doi: 10.1016/j.pacs.2018.10.005.
  10. M. Erfanzadeh, P. D. Kumavor, and Q. Zhu, “Laser scanning laser diode photoacoustic microscopy system,” Photoacoustics, vol. 9, pp. 1–9, 2018, doi: 10.1016/j.pacs.2017.10.001.
  11. H. Zhong, T. Duan, H. Lan, M. Zhou, and F. Gao, “Review of low-cost photoacoustic sensing and imaging based on laser diode and light-emitting diode,” Sensors (Switzerland), vol. 18, no. 7, pp. 20–22, 2018, doi: 10.3390/s18072264.
  12. M. Xu and L. V. Wang, “Photoacoustic imaging in biomedicine,” Rev. Sci. Instrum., vol. 77, no. 4, 2006, doi: 10.1063/1.2195024.
  13. A. Fatima et al., “Review of cost reduction methods in photoacoustic computed tomography,” Photoacoustics, vol. 15, no. May, p. 100137, 2019, doi: 10.1016/j.pacs.2019.100137.
  14. M. Filippi et al., “Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation,” J. Biophotonics, no. December, 2019, doi: 10.1002/jbio.201800035.
  15. Wang and Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nat. Methods, vol. 13, no. 8, pp. 627–638, 2016, doi: 10.1038/nmeth.3925.
  16. F. Gao et al., “An analytical study of photoacoustic and thermoacoustic generation efficiency towards contrast agent and film design optimization,” Photoacoustics, vol. 7, pp. 1–11, 2017, doi: 10.1016/j.pacs.2017.05.001.
  17. D. Wu, L. Huang, M. S. Jiang, and H. Jiang, “Contrast agents for photoacoustic and thermoacoustic imaging: A review,” Int. J. Mol. Sci., vol. 15, no. 12, pp. 23616–23639, 2014, doi: 10.3390/ijms151223616.
  18. G. P. Luke, D. Yeager, and S. Y. Emelianov, “Biomedical applications of photoacoustic imaging with exogenous contrast agents,” Ann. Biomed. Eng., vol. 40, no. 2, pp. 422–437, 2012, doi: 10.1007/s10439-011-0449-4.
  19. P. K. Upputuri and M. Pramanik, “Recent advances in photoacoustic contrast agents for in vivo imaging,” Wiley Interdiscip. Rev. Nanomedicine Nanobiotechnology, vol. 12, no. 4, pp. 1–23, 2020, doi: 10.1002/wnan.1618.
  20. Q. Fu, R. Zhu, J. Song, H. Yang, and X. Chen, “Photoacoustic Imaging: Contrast Agents and Their Biomedical Applications,” Adv. Mater., vol. 31, no. 6, pp. 1–31, 2019, doi: 10.1002/adma.201805875.
  21. A. B. E. Attia et al., “A review of clinical photoacoustic imaging: Current and future trends,” Photoacoustics, vol. 16, no. November, p. 100144, 2019, doi: 10.1016/j.pacs.2019.100144.
  22. S. J. Arconada-Alvarez, J. E. Lemaster, J. Wang, and J. V. Jokerst, “The development and characterization of a novel yet simple 3D printed tool to facilitate phantom imaging of photoacoustic contrast agents,” Photoacoustics, vol. 5, pp. 17–24, 2017, doi: 10.1016/j.pacs.2017.02.001.
  23. A. Hariri, J. Lemaster, J. Wang, A. K. S. Jeevarathinam, D. L. Chao, and J. V. Jokerst, “The characterization of an economic and portable LED-based photoacoustic imaging system to facilitate molecular imaging,” Photoacoustics, vol. 9, pp. 10–20, 2018, doi: 10.1016/j.pacs.2017.11.001.
  24. National Center for Biotechnology Information, “Methyl red,” 2021. https://pubchem.ncbi.nlm.nih.gov/compound/Methyl-red (accessed Mar. 02, 2021).
  25. M. Janna, ““Aplikasi Sistem Pencitraan Fotoakustik berbasis Laser Dioda dan Mikrofon Kondenser untuk Mencitrakan Jaringan Biologis dengan Agen Kontras Pewarna Sintetik,” Universitas Gadjah Mada, Yogyakarta, 2021.
  26. R. Widyaningrum, Mitrayana, R. S. Gracea, D. Agustina, M. Mudjosemedr, and H. M. Silalahi, “The Influence of Diode Laser Intensity Modulation on Photoacoustic Image Quality for Oral Soft Tissue Imaging,” J. Lasers Med. Sci., vol. 11, no. 4, pp. S92–S100, 2020, doi: 10.34172/JLMS.2020.S15.
  27. A. A. Supriyanto, R. M. Suhendar, A. Supendi, P. T. Mekatronika, and P. E. Indorama, “Kalibrasi Alat Ukur Pressure Gauge Sistem Kontrol Level Pada Flashtank 5 Calender,” J. Elektra, vol. 3, no. 1, pp. 20–25, 2018.
  28. D. N. Myers, Innovations in Monitoring With Water-Quality Sensors With Case Studies on Floods, Hurricanes, and Harmful Algal Blooms, 1st ed., vol. 11, no. 2014. Elsevier Inc., 2019.
  29. T. Veuthey, G. Herrera, and V. I. Dodero, “Dyes and stains: from molecular structure to histological application,” pp. 91–112, 2014.
  30. T. Rahmawati, Y. Apriyadi, and Mamay, “Utilization of 1% of Methylene Blue in Staining Histopathological Preparations At Anatomic Pathology Laboratory,” Indones. J. Med. Lab. Sci. Technol., vol. 2, no. 2, pp. 93–100, 2020, doi: 10.33086/ijmlst.v2i2.1563.
  31. A. Lejoy, R. Arpita, B. Krishna, and N. Venkatesh, “Methylene Blue as a Diagnostic Aid in the Early Detection of Potentially Malignant and Malignant Lesions of Oral Mucosa,” Ethiop. J. Health Sci., vol. 26, no. 3, pp. 201–208, 2016.
  32. G. Muthuraman and T. T. Teng, “Extraction of methyl red from industrial wastewater using xylene as an extractant,” Prog. Nat. Sci., vol. 19, no. 10, pp. 1215–1220, 2009, doi: 10.1016/j.pnsc.2009.04.002.

DOI: https://doi.org/10.13057/ijap.v12i2.57219

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