Karakteristik Antibakteri Nanoceria-Kitosan yang disintesis menggunakan Metode Presipitasi

Muhammad Danu Rizkia, Iis Nurhasanah

Abstract

CeO2 nanoparticles (nanoceria) is a material that posseses antibacterial activity. In this study, nanoceria was combined with chitosan to increase its antibacterial property. The formation of nanoceria-chitosan was analyzed using X-ray diffractometer, Fourier Transform Infrared (FTIR) and UV-Vis Spectrophotometer. The antibacterial activity of nanoceria-chitosan was tested using the disc diffusion method on Escherichia coli bacteria. X-ray diffraction pattern analysis showed that nanoceria-chitosan has a cubic structure. The incorporation of chitosan in nanoceria was identified at a wave number of 2919 cm-1 from the FTIR spectrum. Nanoceria-chitosan showed a smaller crystallite size, a larger energy band gap and better antibacterial activity than nanoceria.

Keywords

Antibacterial, Ceria, Chitosan, Nanoparticles

Full Text:

PDF

References

  1. Liu, J., Wu, D., Zhu, N., Wu, Y., & Li, G. 2021. Antibacterial mechanisms and applications of metal-organic frameworks and their derived nanomaterials. Trends in Food Science & Technology, 109, 413–434. https://doi.org/10.1016/ j.tifs.2021.01.012
  2. Alpaslan, C., Geilich, B.M., Yazici, H., & Webster, T.J. 2017. pH-Controlled Cerium Oxide Nanoparticle Inhibition of Both Gram-Positive and Gram-Negative Bacteria Growth. Scientific Reports, 7, 45859. DOI: 10.1038/srep45859
  3. Usman, M.S., Ezzat el Zowalaty, M., Shameli, K., Zainuddin, N., Salama, M., & Ibrahim, N.A. Synthesis, characterization, and antimicrobial properties of copper nanoparticles. International Journal of Nanomedicine, 8, 4467-4479. doi: 10.2147/IJN.S50837
  4. Viet, P. Van, Sang, T.T., Ho, N., Bich, N. & Thi, C.M. 2018. An improved green synthesis method and Escherichia coli antibacterial activity of silver nanoparticles. Journal of Photochemistry & Photobiology B: Biology, 3, 23-30. https://doi.org/10.1016/j.jphotobiol.2018.04.002
  5. Latif, M.M, Anwar-ul- Haq, Amin, F., Ajaz-un-Nabi, M., Khan, I.U., & Sabir, N. 2021. Synthesis and antimicrobial activities of Manganese (Mn) and iron (Fe) co-doped Cerium dioxide (CeO2) Nanoparticles. Physica B, 600, 412562. https://doi.org/10.1016/j.physb.2020.412562
  6. Khadar, Y.A.S., Balamurugan, A., Devarajan, V.P., Subramanian, R. & Kumar, S.D. 2019. Synthesis, characterization and antibacterial activity of cobalt doped cerium oxide (CeO2:Co) nanoparticles by using hydrothermal method. Journal of Materials Research and Technology, 8(1), 267-274. https://doi.org/ 10.1016/j.jmrt.2017.12.005
  7. Senthilkumar, R.P., Bhuvaneshwari, V., Ranjithkumar, R., Sathiyavimal, S., Malayaman, V., & Chandarshekar, B. 2017. Synthesis, characterization and antibacterial activity of hybrid chitosan-cerium oxide nanoparticles-as a bionanomaterials. International Journal of Biological Macromolecules, 16, 34-41. http://dx.doi.org/10.1016/j.ijbiomac.2017.03.139
  8. Surendra, T. V., & Roopan, S.M. 2016. Photocatalytic and antibacterial properties of phytosynthesized of CeO2 NPs using Moringa oleifera peel extract. JPB, 16, 67-71. DOI: 10.1016/j.jphotobiol.2016.05.019
  9. Arumugam, A. Karthikeyan, C., Hameed, A.S.H., Gopinath, K., Gowri, S. & Karthika, V. 2015. Synthesis of cerium oxide nanoparticles using Gloriosa superba L. leaf extract and their structural, optical and antibacterial properties. Materials Science and Engineering C, 49, 408–415. https://doi.org/10.1016/j.msec.2015.01.042
  10. Zhang, M., Zhang, C., Zhai, X., Luo, F., Du, Y., & Yan, C. 2019. Antibacterial mechanism and activity of cerium oxide nanoparticles. Science China Materials, 62(11), 1727-1739.
  11. Bazhukovaa, I.N, Sokovnina, S.Y., Ilvesb, V.G., Myshkinaa, A.V., Vazirova, R.A., Pizurovac, N., dan Kasyanovaa, V.V. 2019. Luminescence and optical properties of cerium oxide nanoparticles. Optical Materials, 92, 136–142. https://doi.org/10.1016/j.optmat.2019.04.021
  12. Ingle, A.P., Duran, N., & Rai M. 2014. Bioactivity, mechanism of action, and cytotoxicity of copper-based nanoparticles: A review. Applied Microbiology and Biotechnology, 98, 1001–1009. DOI 10.1007/s00253-013-5422-8
  13. Asharani, P.V., Low Kah Mun G, & Hande M.P. 2008. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano, 3, 279–290. https://doi.org/10.1021/nn800596w
  14. George, S. Pokhrel, S., Xia, T., Gilbert, B., Ji, Z. Schowalter, M., Rosenauer, A., Damoiseaux, R., Bardley, K.A., Madler, L., & Nel, A.E. 2010. Use of a rapid cytotoxicity screening Approach to engineer a safer Zinc Oxide nanoparticle through Iron doping. ACS Nano, 4, 15–29. https://doi.org/10.1021/nn901503q
  15. De Marzi, L., Monaco, A., & De Lapuente, J. 2013. Cytotoxicity and genotoxicity of ceria nanoparticles on different cell lines in vitro. International Journal of Molecular Sciences, 14, 3065–3077. doi: 10.3390/ijms14023065
  16. Fang, X. & Song, H., 2018. Synthesis of cerium oxide nanoparticles loaded on chitosan for enhanced auto-catalytic regenerative ability and biocompatibility for the spinal cord injury repair. Journal of Photochemistry & Photobiology, B: Biology, 18, 74-87. https://doi.org/10.1016/j.jphotobiol.2018.11.016
  17. Nurhasanah, I., Safitri, W., Arifin, Z., Subagio, A., & Windarti, T. 2018. Antioxidant activity and dose enhancement factor of CeO2 nanoparticles synthesized by precipitation method. IOP Conference Series: Materials Science and Engineering, 432, 012031. doi:10.1088/1757-899X/432/1/012031
  18. Sifontes, A.B., Gonzales, G., Ochoa, J.L., Tovar, L.M., Zoltan, T., & Canizales, E. 2011. Chitosan as template for the synthesis of ceria nanoparticles. Materials Research Bulletin, 46. 1794-1799. https://doi.org/10.1016/ j.materresbull.2011.07.049.
  19. Zhang, L., Zhu, T., Liu, X., & Zhang, W. 2016. Simultaneous oxidation and adsorption of As(III) from water by cerium modified chitosan ultrafine nanobiosorbent. Journal of Hazardous Materials, 308, 1–10. https://doi.org/10.1016/j.jhazmat.2016.01.015
  20. Truffault, L., Ta, M.T., Devers, T., Konstantinov, K., Harel, V., Simmonard, C., Andreazza, C., Nevirkovets,I.P., Pineuau, A., Veron, O., & Blondeau, J.P. 2010. Application of nanostructured Ca doped CeO2 for ultraviolet filtration. Materials Research Bulletin, 45, 527-535. https://doi.org/10.1016/j.materresbull.2010.02.008
  21. Rajan, A.R., Vilasb, V., Rajanc, A., Johna, A., & Philipa, D. 2020. Synthesis of nanostructured CeO2 by chemical and biogenic methods: Optical properties and bioactivity. Ceramics International, 46(9), 14048-14055. https://doi.org/10.1016/j.ceramint.2020.02.204

DOI: https://doi.org/10.13057/ijap.v13i1.55546

Refbacks

  • There are currently no refbacks.