Purity Testing of Cultivar Melon DNA Level (Cucumis melo L.) “Gama Melon Basket” using Surface Plasmon Resonance (SPR) Based on Silver Nanoparticle

Thoyibi T, Muhammad Arifin, Kamsul Abraha

Abstract

DNA level of purity has been tested by observing the phenomenon of surface plasmon resonance (SPR) in silver film + silver (Ag) nanoparticles system as DNA sensor using Kretschmann configuration. Surface plasmon resonance (SPR) phenomenon observed in the resonance condition of reflectance spectrum vary with incident angle of laser, beamed toward prism. System is built as a model for DNA purity test instrument that give higher accuracy. DNA purity testing was conducted by placing DNA layer with various ratio on prism in order to get the angle shift value in each sample as a result. Purity level of DNA indicates the DNA quality based on the ratio between the amount of pure DNA compared to the amount of impurity materials (protein). After the deposition of DNA in ratio 1,774, 1,838 and 1,916 SPR angle shifted ,  and  respectively. Characteristic differences of DNA with different purity (ratio) are shown by reflectance value that is getting higher at a smaller ratio, indicates that in the higher DNA ratio the angle shift is getting smaller. SPR angle shift which is occurred before and after DNA deposition shows the sensitivity of SPR as DNA sensor.

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References

N. Huda, Perakitan dan perbandingan karakter fenotip buah Melon (Cucumis melo L.) kultivar Gama Melon Basket dengan kultivar melon komersial, Skripsi S1, Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta, 2009.

K. Semagn, Å. Bjørnstad and M.N. Ndjiondjop, An overview of molecular marker methods for plants, African Journal of Biotechnology, vol. 5, no. 25, 2006, pp. 2540-2568.

L.R. DeBoer, R.J. Sobieski and S.S. Crupper, Isolation and restriction endonuclease digestion of onion DNA in the junior college-high school biology laboratory, Bioscene, vol. 26, no. 3, 2000, pp. 15-17.

K.K. Vinod, Total genomic DNA extraction, quality check and quantitation, Proceedings of the Training Programme on “Classical and modern Plant Breeding Techniques-A Hands on Training”, Centre for Plant Breeding and Genetic, Tamil Nadu Agricultural University, Coimbatore, India, 2004, pp. 109-121.

D.R Shankaran, K.V. Gobi and N. Miura, Recent advancement in surface plasmon resonance immunosensors for detection of smallmolecules of biomedical, food and environmental interest. Art,Science and Technology Center for Cooperative Research, Kyushu University, Kasuga-shi, Fukuoka, Japan, 2006.

H. Raether, Surface plasmons and smooth and rough surfaces and on grating, Springer Tracts in modern physics, vol. 111, 1988, chap. 2

N.J. Tao, S. Boussaad, W.L. Huang, R.A. Arechabaleta and J. D’Agnese, High resolution surface plasmon resonance spectroscopy, Review of Scientific Instruments, vol.70, 1999, pp. 4656-4660.

C.H. Liao, C.M. Lee, L.B. Chang, Effect of a metal film and prism dielectric on properties of surface plasmon resonance on a multilayer system, Japanese Journal of Applied Physics, vol 36, no. 12, 1999, pp. 1105-1111.

DOI: https://doi.org/10.13057/ijap.v5i01.254

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