PENGEMBANGAN MODEL MATEMATIKA PROSES SIMULTAN ANTARA SAKARIFIKASI DAN FERMENTASI PADA PRODUKSI POLYHYDROXYBUTYRATE

Margono Margono

Abstract

One of the amylolytic bacteria that are able to accumulate polyhydroxybutyrate (PHB) intracellularly is Bacillus sp. However, the ability of these bacteria to synthesize PHB is unknown so further research is still needed. This study aimed to develop a mathematical model of the fermentation process that can be used as a tool for process optimization in the context of the production of PHB using amylolytic bacterium Bacillus sp. and substrate tapioca. Mathematical model is based on a review of simultaneous process between the saccharification and fermentation. Mathematical model consists of 5 simultaneous ordinary differential equations and 15 kinetics parameters. Parameters of the mathematical models was determined by matching the data (data fitting) of experiments and simulations using the genetic algorithm method and MATLAB programming language. The data was obtained from the experiments of  batch process using 1000 mL of shaken flask with working volume of 250 mL. Incubation was performed in an incubator shaker at room temperature, the speed of 130 rpm, and initial pH 7.0. The values of the parameters were μopt 0.037 1/hour, Vm 1,235 1/hr , Km 60.654 g / L, the cell yield (YRSg) 0.170 g dry weight / g glucose and product yield (YPSg) 0.032 g PHB / g glucose.

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DOI: https://doi.org/10.20961/ekuilibrium.v10i2.49638

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