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Banana stem is a biomass that contain high cellulose that can be converted into glucose through hydrolysis reaction. This research purposes were to understand the influence of any kind of acid and its concentration for banana leaves cellulose hydrolysis and to gain the kinetic data or the reaction rate constant (k) of banana leaves hydrolysis reaction. The constant variables in this research were mass of banana leaves (25 gram), mass ratio of solid to solvent (1:10) and cooking temperature (100 oC). The observated variables were kind of acid (H2SO4 and HCl) in each concentration of 1.0 N; 1.5 N; 2.0 N; 2.5 N; 3.0 N. The glucose samples were then analyzed using refractometer. Datas showed that at higher acid concentration and longer reaction time, the higher glucose concentration was formed. Datas also showed that HCl will deliver higher glucose concentration than H2SO4. The maximum operation condition was gained at 2 N HCl concentration. By assuming the order of hydrolysis reaction was one, the reaction rate constant were in the range of 0.000182 – 0.0043/minute and 0.00209 – 0.0066/minute for H2SO4 and HCl respectively.
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