BIODELIGNIFIKASI BATANG JAGUNG DENGAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium

Abstract

The crisis of energy and enviromental problems has led the inovation of the paper manufacturing process that enviromentall- friendly and energy saving. Biological systems can be used for this purposed. The most commonly utilized fungus is the white-rot fungus Phanerochaete chrysosporium. The goal of this research is to determine the lignin degradation in the corn stalk using Phanerochaete chrysosporium. Dried corn stalk with 40 mesh size was soaked in the medium culture, adding with glucose and the pH was adjust at 4. These corn stalk were then sterilized in the autoclaf at 121 oC. Inoculation was done by adding 5 mL fungus suspension. Six flask containing the stalk were placed in the incubator at 38 oC. The culture then incubated for 30 days. The lignin and cellulose content were analyzed every 5 days. A control witrhout adding fungus was treated with same condition. The results show that the longer incubation time, the lower lignin content. At 30 days incubation, 81,4% of lignin degradation were achieved. The degradation of lignin occured together with degradation of cellulose but the degradation of cellulose is less then the lignin. At 30 days incubation, 22,3% of cellulose degradation were achieved.