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Uji Toleransi Logam Berat Bakteri Hidrokarbonoklastik dan Uji Kemampuan Micrococcus sp. LII61 dalam Menurunkan Kromium (Cr VI), Tembaga (Cu II), Seng (Zn II)


 
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1. Title Title of document Uji Toleransi Logam Berat Bakteri Hidrokarbonoklastik dan Uji Kemampuan Micrococcus sp. LII61 dalam Menurunkan Kromium (Cr VI), Tembaga (Cu II), Seng (Zn II)
 
2. Creator Author's name, affiliation, country Achmad Zainal Abidin; Universitas Airlangga, Mulyorejo, Surabaya, Indonesia; Indonesia
 
2. Creator Author's name, affiliation, country Elga Renjana; Universitas Airlangga, Mulyorejo, Surabaya, Indonesia
 
2. Creator Author's name, affiliation, country Fatimah Fatimah; Universitas Airlangga, Mulyorejo, Surabaya, Indonesia
 
2. Creator Author's name, affiliation, country Ni’matuzahroh Ni’matuzahroh; Universitas Airlangga, Mulyorejo, Surabaya, Indonesia; Indonesia
 
3. Subject Discipline(s) Microbiology, bioremediation
 
3. Subject Keyword(s) Hydrocarbon-degrading bacteria, heavy metals, Micrococcus sp. LII61, tolerance
 
4. Description Abstract This research aimed to know: (1) tolerance level of hydrocarbon-degrading bacteria collected from Biology Laboratory of Airlangga University i.e. Acinetobacter sp P2(1) Bacillus subtilis 3KP, Micrococcus sp. LII61, Pseudomonas putida T1(8), at various concentration of heavy metal chromium (Cr6+), copper (Cu2+), zinc (Zn2+), and combination of that three metals; (2) growing ability of selected bacteria in lowering metals concentration; and (3) reduction percentage of metal concentration by selected bacteria. Tolerance test was performed using streak plate method on two types of solid mediums, i.e. Mineral Salt Medium (MSM) agar and Mueller-Hinton Agar (MHA) with a stratified concentration and 72 hours of incubation time. Bacterial growth and metal concentration reduction test were carried out in liquid medium containing single metal or combined metals. Viability of bacteria was calculated using Total Plate Count (TPC) method. Logarithmic of TPC were analyzed statistically using Brown-Forsythe test followed by Games-Howell. The results showed: (1) All bacteria was resistant to either single heavy metal or combined metals on both medium. However, Micrococcus sp. LII61, had the highest tolerance compared to the other three bacteria, which was able to grow at Zn+2 1000 ppm in both medium. Micrococcus sp. LII61, was the selected bacteria to be tested for its ability to reduce heavy metal by single heavy metal and combined metals for 3 and 7 days of incubation time. (2) Ability levels of Micrococcus sp. LII61, to grow in some heavy metals was Zn2+ > Cu2+ > Cr6+ consecutively. (3) Ability levels of Micrococcus sp. LII61, in reducing heavy metals was Cu2+ > Zn2+ > Cr6+ consecutively. Percentage decrease of Cu2+ in single metal was 66.15%, while in combination metals was 54.25% at 3 days incubation. Percentage decrease of Cu2+ after seven day incubation in single metals and combination metals were 90.13% and 74.72% respectively.
 
5. Publisher Organizing agency, location Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un
 
6. Contributor Sponsor(s)
 
7. Date (YYYY-MM-DD) 2019-02-15
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://jurnal.uns.ac.id/bioedukasi/article/view/27414
 
10. Identifier Digital Object Identifier (DOI) https://doi.org/10.20961/bioedukasi-uns.v12i1.27414
 
11. Source Title; vol., no. (year) Bioedukasi: Jurnal Pendidikan Biologi; Vol 12, No 1 (2019): Bioedukasi: Jurnal Pendidikan Biologi
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2019 Bioedukasi: Jurnal Pendidikan Biologi