Efek Radiasi Sinar Gamma pada Pertumbuhan Vegetatif Anggrek Vanda Hibrid

Sri Hartati, Abednego Widya Setiawan, Trijono Djoko Sulistyo

Abstract

Orchids are one of the most important ornamental plants in the world. The existence of natural orchids in their habitat is approaching extinction rate, so it is necessary to increase the diversity of orchids by crossing them. The Vanda orchid is one of the famous orchids in Indonesia because of its uniqueness. Vanda orchids have a very abundant diversity, so they can increase the potential of orchid plants. This study used a hybrid orchid plant Vanda celebica x Vanda dearei irradiated by Gamma-rays with a dose of 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray, and 50 Gray. The data obtained from the observations will be analyzed using descriptive methods by observing each plant in various doses of Gamma-ray radiation compared with control treatment and presented in the form of boxplot images. The irradiation dose of hybrid Vanda orchids (Vanda celebica x Vanda dearei) increased the diversity in the parameters of plant height and leaf width. The application of a dose of Gamma-ray irradiation did not cause a significant interaction with the variable number of leaves. The diversity value decreased in the parameters of leaf length and number of roots. Application doses of Gamma-ray irradiation of 10, 20, and 30 Gray can change the leaves into grooves compared to control plants. Mutant plants had lower chlorophyll content than control plants (0 Gray).

Keywords

Boxplot; Vanda celebica; Vanda dearie; Diversity; Mutation

Full Text:

PDF(ID)

References

Billore V, Mirajkar SJ, Suprasanna P, Jain M. 2019. Gamma irradiation induced effects on in vitro shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of Dendrobium sonia orchid. Biotechnol Reports. 22:e00343. https://doi.org/10.1016/j.btre.2019.e00343.

Dehgahi R, Joniyasa A. 2017. Gamma Irradiation-Induced Variation in Dendrobium Sonia-28 OrchidProtocorm-Like Bodies (PLBs). Fungal Genomics Biol. 07(02):1000151. https://doi.org/10.4172/2165-8056.1000151.

Deshmukh P, Malode S. 2017. Review of research on Dendrobium sonia-28, a hybrid from Orchidacea family and mutation as somaclonal variation. Int J Biosci. 10(06):29–47. https://doi.org/10.12692/ijb/10.6.29-47.

Devy L, Sastra DR. 2006. Pengaruh radiasi sinar Gamma terhadap kultur in Vitro tanaman jahe. J Sains dan Teknol Indones. 8(1):7–14.

Fathin TS, Hartati S, Yunus A. 2021. Diversity induction with gamma ray irradiation on Dendrobium odoardi orchid. IOP Conf Ser Earth Environ Sci. 637(1):012035. https://doi.org/10.1088/1755-1315/637/1/012035.

Gaur AK, Singh I, Singh S, Reddy KS. 2018. Studies on effects of Gamma ray doses on germination in pigeonpea [Cajanus cajan (L.) Millspaugh] under laboratory and field conditions Studies on effects of Gamma ray doses on germination in pigeonpea [Cajanus cajan (L.) Millspaugh] under laborat. Int J Chem Stud. 6(4):1975–1977.

Handayati W. 2013. Perkembangan pemuliaan mutasi tanaman hias di Indonesia. J Ilm Apl Isot dan Radiasi. 9(1):67–80.

Hartati S, Darsana L. 2015. Karakterisasi anggrek alam secara Morfologi dalam rangka pelestarian Plasma Nutfah. J Agron Indones. 43(2):133–139. https://doi.org/10.24831/jai.v43i2.10419.

Hartati S, Muliawati ES, Pardono P, Cahyono O, Yuliyanto P. 2019. Morphological characterization of Coelogyne spp for germplasm conservation of orchids. Rev Ceres. 66(4):265–270. https://doi.org/10.1590/0034-737x201966040004.

Hartati S, Yunus A, Nugroho F. 2017. Keragaan anggrek persilangan ♀ Vanda celebica X ♂ Vanda dearei hasil iradiasi sinar Gamma. Agrotechnology Res J. 1(1):7–12. https://doi.org/10.20961/agrotechresj.v1i1.18846.

Indahsari D, Saputro TB. 2018. Analisis morfologi dan profil protein kedelai varietas grobogan hasil iradiasi pada kondisi cekaman genangan. J Sains dan Seni ITS. 7(2):2337–3520. https://doi.org/10.12962/j23373520.v7i2.37346.

Karyanti K. 2017. Pengaruh beberapa jenis sitokinin pada multiplikasi tunas anggrek Vanda douglas secara in vitro. J Bioteknol dan Biosains Indones. 4(1):36–43. https://doi.org/10.29122/jbbi.v4i1.2200.

Kasutjianingati K, Firgiyanto R. 2018. Characterization of morphology from orchid Vanda sp. as a genetic information source for preservation and agribusiness of orchids in Indonesia. IOP Conf Ser Earth Environ Sci. 207(1):012006. https://doi.org/10.1088/1755-1315/207/1/012006.

Lestari EP, Yunus A, Sugiyarto S. 2018. Pengaruh morfologi Anggrek Dendrobium sylvanum dan Anggrek Phalaenopsis sp. dengan pemberian iradiasi sinar Gamma. In: Khasanah LU, Septariani DN, Wati AK, Sanjaya AP, editor. Peran keanekaragaman hayati dalam mendukung Indonesia sebagai lumbung pangan dunia. Prosiding Seminar Nasional Dalam Rangka Dies Natalis UNS Ke 42 Tahun 2018; 18-19 April 2018; Surakara, ID. Vol. 2. Surakarta (ID): FP UNS. hal. 143–152.

Mujafar S, Mishra S, Deoda VS, Moinuddin S, Mutakim S. 2013. Orchid species diversity of East Nimar. Int J Plant, Anim Environtmental Sci. 3(4):222–230.

Mujar EK, Sidik NJ, Sulong NA, Jaapar SS, Othman MH. 2014. Effect of low Gamma radiation and methyl jasmonate on Vanilla planifolia tissue culture. Int J Pharm Sci Rev Res. 27(1):163–167.

Nurmansyah, Alghamdi SS, Migdadi HM. 2020. Morphological diversity of faba bean (Vicia faba L.) M2 mutant populations induced by Gamma radiation and diethyl sulfate. J King Saud Univ - Sci. 32(2):1647–1658. https://doi.org/10.1016/j.jksus.2019.12.024.

Nurrachmamila PL, Saputro TB. 2017. Analisis daya perkecambahan padi (Oryza sativa L.) varietas Bahbutong hasil iradiasi. J Sains dan Seni ITS. 6(2):2337–2342. https://doi.org/10.12962/j23373520.v6i2.23952.

Oktavina ZS. 2011. Pengaruh iradiasi sinar Gamma terhadap pertumbuhan anggrek hibrid Dendrobium schulerii x may neal wrap secara in vitro [skripsi]. Jakarta (ID): Universitas Islam Negeri Syarif Hidayatullah.

Rahman QK, Aisyah SI. 2018. Induksi mutasi fisik pada paku bintik (Microsorum punctatum) melalui Iradiasi Sinar Gamma. Bul Agrohorti. 6(3):422–429. https://doi.org/10.29244/agrob.v6i3.21112.

Shidiqy HA, Wahidah BF, Hayati N. 2018. Karakterisasi morfologi Anggrek (Orchidaceae) di hutan kecamatan Ngaliyan Semarang. Al-Hayat J Biol Appl Biol. 1(2):94–98. https://doi.org/10.21580/ah.v1i2.3761.

Singh PK, Sadhukhan R, Kumar V, Sarkar HK. 2019. Gamma rays and EMS induced chlorophyll mutations in grasspea (Lathyrus sativus L.). Int J Bio-resource Stress Manag. 10(2):113–118. https://doi.org/10.23910/ijbsm/2019.10.2.1940b.

Srivastava D, Gayatri MC, Sarangi SK. 2018. In vitro mutagenesis and characterization of mutants through morphological and genetic analysis in orchid Aerides crispa Lindl. Indian J Exp Biol. 56(6):385–394.

Taryono. 2016. Pengantar bioteknologi untuk pemuliaan tanaman. Yogyakarta (ID): Gadjah Mada University Press.

Togatorop ER, Aisyah SI, Damanik MRM. 2016. Pengaruh mutasi fisik iradiasi sinar Gamma terhadap keragaman genetik dan penampilan Coleus blumei. J Hortik Indones. 7(3):187–194. https://doi.org/10.29244/jhi.7.3.187-194.

Yadav V. 2016. Effect of Gamma radiation on various growth parameters and biomass of Canscora decurrens Dalz. Int J Herb Med. 4(5):109–115.

Yu Y, Oo N, Su C, Kyaw KT. 2017. Extraction and determination of chlorophyll content from microalgae. Ijarp. 1(5):298–301.

Yunita R, Khumaida N, Sopandie D, Mariska I. 2016. Pengaruh iradiasi sinar gama terhadap pertumbuhan dan regenerasi Kalus padi varietas Ciherang dan Inpari 13. J AgroBiogen. 10(3):101–108. https://doi.org/10.21082/jbio.v10n3.2014.p101-108.

Refbacks

  • There are currently no refbacks.