Effects of Leucaena Leaf Extract as a Liquid Organic Biostimulant and Harvest Timing on Yield and Seed Quality of Sesame (Sesamum indicum) in Dryland Systems
Abstract
Sesame productivity and seed quality in dryland systems are often constrained by low soil fertility and inappropriate harvest timing. This study aimed to evaluate the effects of Leucaena leucocephala leaf extract applied as a liquid organic biostimulant and different harvest timings on growth, yield, seed quality, and oil content of sesame cultivated under dryland conditions. The experiment was conducted using a factorial randomized complete block design with four extract doses (0, 100, 200, and 300 mL plant⁻¹) and three harvest timings (95, 102, and 109 days after planting). Vegetative growth parameters, yield components, seed physical and physiological quality, and oil content were measured and analyzed. The results showed that increasing extract doses significantly enhanced vegetative growth, capsule formation, seed weight, and total seed yield, with the highest yield obtained at 300 mL plant⁻¹. Harvest timing strongly influenced seed physiological quality and oil accumulation. Seeds harvested at 102 days after planting exhibited the highest germination performance and oil content, whereas earlier or later harvest reduced quality attributes. A moderate extract dose (200 mL plant⁻¹) combined with harvest at 102 days optimized seed quality and oil content, while higher doses favored yield maximization. These findings indicate that synchronizing organic nutrient management with appropriate harvest timing is essential to balance yield and quality in sustainable dryland sesame production systems.
Keywords
Full Text:
PDFReferences
Al-Shammary AAG, Al-Shihmani LSS, Fernández-Gálvez J, Caballero-Calvo A. 2024. Optimizing sustainable agriculture: A comprehensive review of agronomic practices and their impacts on soil attributes. J Environ Manage. 364:121487. doi:10.1016/j.jenvman.2024.121487. https://linkinghub.elsevier.com/retrieve/pii/S0301479724014737.
Alemayehu S, Abera FA, Ayimut KM, Darnell R, Mahroof R, Harvey J, Subramanyam B. 2023. Effects of Storage Duration and Structures on Sesame Seed Germination, Mold Growth, and Mycotoxin Accumulation. Toxins (Basel). 15(1):39. doi:10.3390/toxins15010039. https://www.mdpi.com/2072-6651/15/1/39.
Anyogu A, Somorin YM, Oladipo AO, Raheem S. 2024. Food safety issues associated with sesame seed value chains: Current status and future perspectives. Heliyon. 10(16):e36347. doi:10.1016/j.heliyon.2024.e36347. https://linkinghub.elsevier.com/retrieve/pii/S240584402412378X.
Balouchi H, Soltani Khankahdani V, Piri R, Moradi A, Gholamhoseini M, Heydari SZ, Ahmed LQ, Escobar-Gutiérrez AJ, Dedicova B. 2025. Deciphering the Germination Behavior of Sesame Cultivars: The Interplay of Hydrothermal Time Model Parameters and Seed Fatty Acid Profiles. Plants. 14(22):3422. doi:10.3390/plants14223422. https://www.mdpi.com/2223-7747/14/22/3422.
Baraki F, Hadgu F, Berhe G. 2025. Soil fertility management on sesame (Sesamum indicum L.) production in Northern Ethiopia: A review. Heliyon. 11(2):e41618. doi:10.1016/j.heliyon.2024.e41618. https://linkinghub.elsevier.com/retrieve/pii/S2405844024176499.
Basar NU, Shahid MA, Primo ASB, Kadyampakeni DM. 2025. Synergies between biostimulants and plant nutrients: a review of ecofriendly nutrient management in crop production. Discov Agric. 3(1):150. doi:10.1007/s44279-025-00345-x. https://link.springer.com/10.1007/s44279-025-00345-x.
Berhane A, Abrha B, Worku W, Hadgu G. 2024. Modeling the response of sesame (Sesamum indicum L.) to different soil fertility levels under rain-fed conditions in the semi-arid areas of western Tigray, Ethiopia. Heliyon. 10(17):e36084. doi:10.1016/j.heliyon.2024.e36084. https://linkinghub.elsevier.com/retrieve/pii/S2405844024121159.
Borreani G, Peiretti PG, Tabacco E. 2007. Effect of harvest time on yield and pre-harvest quality of semi-leafless grain peas (Pisum sativum L.) as whole-crop forage. F Crop Res. 100(1):1–9. doi:10.1016/j.fcr.2006.04.007. https://linkinghub.elsevier.com/retrieve/pii/S0378429006001067.
Boutahiri S, Benrkia R, Tembeni B, Idowu OE, Olatunji OJ. 2024. Effect of biostimulants on the chemical profile of food crops under normal and abiotic stress conditions. Curr Plant Biol. 40:100410. doi:10.1016/j.cpb.2024.100410. https://linkinghub.elsevier.com/retrieve/pii/S2214662824000926.
Comini E, Rubiales D, Reveglia P. 2023. Variability of Fatty Acid Composition and Lignan Content in Sesame Germplasm, and Effect of Roasting. ACS Food Sci Technol. 3(10):1747–1758. doi:10.1021/acsfoodscitech.3c00304. https://pubs.acs.org/doi/10.1021/acsfoodscitech.3c00304.
Duri LG, Paradiso R, Di Mola I, Cozzolino E, Ottaiano L, Marra R, Mori M. 2025. Organic Fertilization and Biostimulant Application to Improve Yield and Quality of Eggplant While Reducing the Environmental Impact. Plants. 14(6):962. doi:10.3390/plants14060962. https://www.mdpi.com/2223-7747/14/6/962.
Fajdetić NR, Božić Ostojić L, Benković R, Zima D, Blažinkov M, Mirosavljević K, Popović B, Benković-Lačić T. 2025. Effects of Three Organic Fertilizers and Biostimulants on the Morphological Traits and Secondary Metabolite Content of Lettuce. Horticulturae. 11(11):1288. doi:10.3390/horticulturae11111288. https://www.mdpi.com/2311-7524/11/11/1288.
Gebregergis Z, Baraki F, Fiseseha D. 2024 May 22. Effects of environmental factors and storage periods on sesame seed quality and longevity. CABI Agric Biosci. doi:10.1186/s43170-024-00247-w. http://www.cabidigitallibrary.org/doi/10.1186/s43170-024-00247-w.
Gesch RW, Johnson BL. 2012. Seed moisture at physiological maturity in oilseed and confectionary sunflower hybrids in the northern U.S. F Crop Res. 133:1–9. doi:10.1016/j.fcr.2012.03.012. https://linkinghub.elsevier.com/retrieve/pii/S0378429012001050.
Kabato W, Hailegnaw N, Mutum L, Molnar Z. 2025. Managing soil health for climate resilience and crop productivity in a changing environment. Sci Total Environ. 1000:180460. doi:10.1016/j.scitotenv.2025.180460. https://linkinghub.elsevier.com/retrieve/pii/S004896972502100X.
Kishk AM. 2024. Effect of plant biostimulants, harvesting dates, and storage periods on Sesame (Sesamum indicum L.) seed quality in newly reclaimed soils. Egypt J Agron. 46(2):329–341. doi:10.21608/agro.2024.304521.1464. https://agro.journals.ekb.eg/article_387308.html.
kouighat M, Kettani R, El Fechtali M, Nabloussi A. 2024. Exploring mechanisms of drought-tolerance and adaptation of selected sesame mutant lines. J Agric Food Res. 15:100911. doi:10.1016/j.jafr.2023.100911. https://linkinghub.elsevier.com/retrieve/pii/S2666154323004180.
Langyan S, Yadava P, Sharma S, Gupta NC, Bansal R, Yadav R, Kalia S, Kumar A. 2022. Food and nutraceutical functions of sesame oil: An underutilized crop for nutritional and health benefits. Food Chem. 389:132990. doi:10.1016/j.foodchem.2022.132990. https://linkinghub.elsevier.com/retrieve/pii/S0308814622009529.
Lee J, Jo N-Y, Shim S-Y, Le TYL, Jeong WY, Kwak KW, Choi HS, Lee B-O, Kim S-R, Lee M-G, et al. 2025. Impact of organic liquid fertilizer on plant growth of Chinese cabbage and soil bacterial communities. Sci Rep. 15(1):10439. doi:10.1038/s41598-025-95327-w. https://www.nature.com/articles/s41598-025-95327-w.
Liu Y, Lan X, Hou H, Ji J, Liu X, Lv Z. 2024. Multifaceted Ability of Organic Fertilizers to Improve Crop Productivity and Abiotic Stress Tolerance: Review and Perspectives. Agronomy. 14(6):1141. doi:10.3390/agronomy14061141. https://www.mdpi.com/2073-4395/14/6/1141.
Matthews S, Siddiqui Y, Ali A. 2025. Unleashing the power of bio-stimulants for enhanced crop growth, productivity, and quality: a comprehensive review. J Plant Nutr. 48(4):703–725. doi:10.1080/01904167.2024.2412736. https://www.tandfonline.com/doi/full/10.1080/01904167.2024.2412736.
Moreno L, Santos AF, Tubbs RS, Grey TL, Monfort WS, Lamb MC, Pilon C. 2024. Physiological components of seed quality in runner‐type peanut during seed formation. Agron J. 116(1):189–201. doi:10.1002/agj2.21506. https://acsess.onlinelibrary.wiley.com/doi/10.1002/agj2.21506.
Neumann G, Nawaz F, Weinmann M, Arbona V, Balestrini R, Pagliarani C, Gonzalez-Guzman M. 2024. Editorial: Enhancing sustainable crop production: biostimulants and biotechnological approaches in challenging climates. Front Plant Sci. 15. doi:10.3389/fpls.2024.1534774. https://www.frontiersin.org/articles/10.3389/fpls.2024.1534774/full.
Norouzi Z, Nateghi L, Rashidi L, Movahhed S. 2025. Increasing the bioactive properties of sesame seed meal as a valuable protein source through the enzymatic hydrolysis. Food Biosci. 68:106790. doi:10.1016/j.fbio.2025.106790. https://linkinghub.elsevier.com/retrieve/pii/S2212429225009666.
Oboulbiga EB, Douamba Z, Compaoré-Sérémé D, Semporé JN, Dabo R, Semde Z, Tapsoba FW-B, Hama-Ba F, Songré-Ouattara LT, Parkouda C, et al. 2023. Physicochemical, potential nutritional, antioxidant and health properties of sesame seed oil: a review. Front Nutr. 10. doi:10.3389/fnut.2023.1127926. https://www.frontiersin.org/articles/10.3389/fnut.2023.1127926/full.
Prashar D, Singh A, Dhama V, Singh PK, Kumar M, Kumar S, Pandey D, Verma G. 2025. Effect of Organic and Inorganic Fertilizers on Crop Yield and Soil Fertility: A Comprehensive Review. J Exp Agric Int. 47(2):16–22. doi:10.9734/jeai/2025/v47i23261. https://journaljeai.com/index.php/JEAI/article/view/3261.
Samijan, Minarsih S, Jauhari S, Basuki S, Susila A, Nurwahyuni E, Hindarwati Y, Supriyo A, Aristya VE. 2023. Revitalizing sub-optimal drylands: Exploring the role of biofertilizers. Open Agric. 8(1). doi:10.1515/opag-2022-0214. https://www.degruyter.com/document/doi/10.1515/opag-2022-0214/html.
Sanni GBTA, Ezin V, Chabi IB, Missihoun AA, Florent Q, Hamissou Z, Niang M, Ahanchede A. 2024. Production and achievements of Sesamum indicum industry in the world: Past and current state. Oil Crop Sci. 9(3):187–197. doi:10.1016/j.ocsci.2024.06.006. https://linkinghub.elsevier.com/retrieve/pii/S2096242824000435.
Seadh SE, Badawi MA, EL-Abady MI, EL-Saidy A. 2007. How varieties and harvesting dates of sesame affect seed yield and quality. J Soil Sci Agric Eng. 32(12):10811–10823. doi:10.21608/jssae.2007.201811. https://jssae.journals.ekb.eg/article_201811.html.
Shah IH, Jinhui W, Li X, Hameed MK, Manzoor MA, Li P, Zhang Y, Niu Q, Chang L. 2024. Exploring the role of nitrogen and potassium in photosynthesis implications for sugar: Accumulation and translocation in horticultural crops. Sci Hortic (Amsterdam). 327:112832. doi:10.1016/j.scienta.2023.112832. https://linkinghub.elsevier.com/retrieve/pii/S0304423823010002.
Smith MR, Rao IM, Merchant A. 2018. Source-Sink Relationships in Crop Plants and Their Influence on Yield Development and Nutritional Quality. Front Plant Sci. 9. doi:10.3389/fpls.2018.01889. https://www.frontiersin.org/article/10.3389/fpls.2018.01889/full.
Sofian M, Raksun A, Handayani BS, Zulkifli L. 2025. Effect of NPK Fertilizer and Liquid Organic Fertilizer Lamtoro Leaves (Leucaena leucocephala spp. leucocephala) on The Growth of Cayenne Peppers (Capsicum frutescens L.). J Biol Trop. 25(3):4618–4633. doi:10.29303/jbt.v25i3.10031. http://jurnalfkip.unram.ac.id/index.php/JBT/article/view/10031.
Sripathy K V., Groot SPC. 2023. Seed Development and Maturation. In: Seed Science and Technology. Singapore: Springer Nature Singapore. p. 17–38. https://link.springer.com/10.1007/978-981-19-5888-5_2.
Tang Q, Wang G, Zhao L, Song Z, Li Y. 2023 Dec. Response of yield, root traits and plasticity of nitrogen-efficient cultivars of drip-irrigated rice to a nitrogen environment. J Integr Agric. doi:10.1016/j.jia.2023.12.014. https://linkinghub.elsevier.com/retrieve/pii/S2095311923004616.
Usman S. 2024. Soil and water management perspectives for tropical and dryland areas of Africa. Soil Stud. 13(2):104–118. doi:10.21657/soilst.1601786. http://dergipark.org.tr/en/doi/10.21657/soilst.1601786.
Wang J, Li H, Wang Q, Huang X, Hu W, Wang S, Zhou Z. 2023. Effects of phosphorus application on carbohydrate metabolism in cottonseed kernel during the key development period provided a new insight for phosphorus management in cotton production. Ind Crops Prod. 191:115972. doi:10.1016/j.indcrop.2022.115972. https://linkinghub.elsevier.com/retrieve/pii/S0926669022014558.
Wei J, Zhang X, Li G, Fu K, Yan M, Li Cheng, Li Chunyan. 2025. Phosphorus Alters the Metabolism of Sugars and Amino Acids in Elite Wheat Grains. Plants. 14(20):3152. doi:10.3390/plants14203152. https://www.mdpi.com/2223-7747/14/20/3152.
Yosilia R, Joevira AE, Winandari OP. 2025. The effect of liquid organic fertilizer (lof) derived from leucaena leaves (Leucaena leucocephala) and pineapple peels (Ananas comosus L.) on pak choi (Brassica rapa subsp. chinensis) Growth. J Agrotropika. 24(2):375–386. doi:10.23960/ja.v24i2.10407. https://jurnal.fp.unila.ac.id/index.php/JAT/article/view/10407.
Zhang Y, Wang X, Liu B, Liu Q, Zheng H, You X, Sun K, Luo X, Li F. 2020. Comparative study of individual and Co-Application of biochar and wood vinegar on blueberry fruit yield and nutritional quality. Chemosphere. 246:125699. doi:10.1016/j.chemosphere.2019.125699.
Refbacks
- There are currently no refbacks.



