DAYA ANTIFUNGAL EKSTRAK ETANOL DAUN (Piper aduncum) DAN (Piperomia pellucida) TERHADAP PERTUMBUHAN (C. albicans) SECARA INVITRO

Abstract

Abstract - Sesuruhan plants (Piper aduncum) and messengers (Piperomia pellucida) is a species of Piperaceae familia members. Both of these plant species containing antifungal compounds include: tannins, flavonoids, and saponins. Candida albicans is a yeast causing vaginal discharge disease. Antifungal potential of ethanol extract of the plant, particularly against C. albicans in vitro testing needs. The purpose of this study is to: 1) examine the effect of ethanol extract of leaves sesuruhan in some sort of concentration on the inhibition of growth of C. albicans in vitro; 2) examine the effect of ethanol extract of leaves of messengers in some sort of concentration on the inhibition of growth of C. albicans in vitro; 3) determine the most effective concentration of the ethanol extract of leaves and leaf sesuruhan messengers in some kind of concentration to inhibit the growth of C. albicans in vitro. Extracts of both species was obtained by maceration method with 95% ethanol, followed by sterile filtration and evaporation of the filtrate thus obtained. Filtrate was diluted in 10 concentration levels, namely: 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, and 90%. Testing the two kinds of power antifungal leaf species was conducted using agar diffusion technique of pitting on Sabouraud Dextrose Agar medium that has been inoculated with C. albicans. Measurement of C. albicans growth inhibitory zone performed after an incubation period of 1x24 hours. The results of this study are: 1) There is no effect of ethanol extract of leaves sesuruhan in some sort of concentration on the inhibition of growth of C. albicans in vitro. The higher the concentration of the ethanol extract of the leaves sesuruhan increasing inhibition of growth of C. albicans; 2) There is no effect of ethanol extract of leaves of messengers in some sort of concentration on the inhibition of growth of C. albicans in vitro. The higher the concentration of the ethanol extract of the leaves of messengers increasing inhibition of growth of C. albicans; 3) Ethanol extract of leaves and leaf sesuruhan errand at a concentration of 80% was the most effective concentration to inhibit the growth of C. albicans in vitro.

Keywords: antifungal power, Piper aduncum leaves, leaf Piperomia pellucida, Candida albicans