Alternative Media FOR Bacterial Growth Using a different Source of Carbohidrats

Abstract

The high cost of instant media such as nutrient agar encourage researchers to find an alternative media from raw materials that are easily available and cheap. This study aims to determine of using a source of carbohydrates to used as an alternative media. The source of carbohydrates that can be used is Canna edulis, Dioscorea esculenta, and Maranta arundinaceae and the results were compared with nutrient agar. The sample used are 300 gr of tubers with addition 10 gr of sugar and 15 gr of agar in 1000 ml of destilled water. This study used a Completely Randomized Design (CRD) two factors. The first factor is a type of bacteria: Escherichia coli and Staphylococcus aureus and the second factor is the types of alternative media: Canna edulis, Dioscorea esculenta, and Maranta arundinaceae. The bacteria were inoculated with a spread plate method and incubated at 37 ° C for 24 hours. the parameters are a number of population and the size of the bacterial colonies.The result showed that in Escherichia coli, the highest population is in the Canna edulis (7.56x10⁷CFU/ml), Dioscorea esculenta (6,86x10⁷CFU/ml), nutrient agar media (6.21x10⁷CFU/ml), and the last is Maranta arundinaceae (5.49x10⁷CFU/ml) while in the the Staphylococcus aureus, the highest population is in the Canna edulis (8.17x10⁷CFU/ml), Maranta arundinaceae (5.13x10⁷CFU/ml), Dioscorea esculenta (4.08x10⁷CFU/ml), and the last is nutrient agar media (4.02x10⁷CFU/ml). Based on the size of the bacteria, the best result is in the  Dioscorea esculenta, because the bacterial colonies are large and looks more clear. The results showed that the media of Canna edulis, Dioscorea esculenta, and Maranta arundinaceae can be used as an alternatives media for bacterial growth and the most optimal media is  Dioscorea esculenta.