PENGHAMBATAN EKSTRAK METANOL DAUN DAN BATANG TUMBUHAN MANGROVERhizophoramucronataTERHADAP PERTUMBUHAN BEBERAPA STRAIN BAKTERI Aeromonas hydrophila

Heri Maryanto, Ana Miftahul Janah, Dini Siswani Mulia

Abstract

Aeromonas hydrophila is one of the pathogenic bacteria that attack freshwater fish. These bacteria are also pathogenic in brackish water fish and sea and other aquatic animals. Treatment of this bacterial attack should be pursued by using natural materials that are more effective and environmentally friendly, one of which is Rhizophora mucronata. This study aims to determine the inhibition of methanol extract of leaves and stems of R. mucronata mangrove plant on the growth of several strains of A. hydrophila bacteria. The experiment was conducted using experimental method with Completely Randomized Design (RAL) 3 factor factorial, ie A factor (type of bacterial strain): A1 (GPl-04), A2 (GL-01), A3 (GL-02), A4 (GJ- 01), A5 (GK-01), and A6 (GB-01); B (plant part): B1 (stem) and B2 (leaf); C (concentration of methanol extract R. mucronata): C1 (0% / control), C2 (10%), C3 (20%), and C4 (30%), with 3 replications. The parameters observed were the results of phytochemical screening test by Thin Layer Chromatography (TLC) and bacterial growth inhibition (clear zone). Data of clear zone were analyzed using Analysis of Variance (Anova) and Duncan Multiple Range Test (DMRT) with 5% test level, while the result of phytochemical screening test with TLC was analyzed descriptively qualitative. The results showed that R. mucronata mangrove plants potentially inhibited the growth of A. hydrophila because it contains flavonoids, alkaloids, terpenoids, and tannins. The type of bacterial strain and concentration of R. mucronata methanol extract had significant effect (P0 <0,05) on the inhibitory zone diameter but the plant part and interaction of the three factors, ie strains of bacteria, plant part, and concentration of R. mucronata methanol extract were not significantly different P> 0.05). Plant parts of both stems and leaves have the same potential as a natural bactericidal material. Bacteria A. hydrophila GL-01 strain was most sensitive, whereas GK-01 strain was most resistant to R. mucronata methanol extract. 10% concentration is an effective and efficient concentration in inhibiting the growth of A.hydrophila bacteria.

Keywords

Aeromonas hydrophila, extract of methanol, mangrove, natural bactericide, Rhizophora mucronata,strain of bacteria

Full Text:

PDF

References

Erawati, C.I & Marsoedi. 2004. Pengaruh pemberian Perasan Kasar Daun pepaya (Carica papaya) dengan Dosis Yang Berbeda terhadap Ikan Mas (Cyprinus carpio) yang Terinfeksi Bakteri Aeromonas hydrophila. Jurnal Penelitian Perikanan 7 (2).

Harikrishnan, R. & C. Balasundaram. 2005. Modern Trends in Aeromonas hydrophilaDisease Management with Fish. Review in Fisheries Science. 13: 281-320.

Janda, J.M. & Abbott, S.L. 2010. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clinical Microbiology Reviews. 23: 35-73

Kamiso, H.N. 2004. Status Penyakit Ikan dan Pengendaliannya di Indonesia. Prosiding Seminar Nasional Penyakit Ikan dan Udang IV, Purwokerto, 18-19 Mei 2004.

Nababan, E., Dwi, S., & Febrina, A. 2012. Potensi Ekstrak Kulit Batang Rhizophora Mucronata Sebagai Antibakteri Untuk Menanggulangi Serangan Bakteri Aeromonas Hydrophila Pada Benih Ikan Gurami (Osphronemus Gouramy). Sumatra : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian, Universitas Sumatera Utara,

Rohaeti, E., Batubara, I., Lieke, A., & Darusman, L. K. 2010. Potensi Ekstrak Rhizophora sp. Sebagai Inhibitor Tirosinase. Prosiding Semnas Sains III. IPB, Bogor, 13 November 2010. p. 196-201.

Suciati, A., Wardiyanto & Sumino. 2012. Efektifitas Esktrak Daun Rhizophora mucronata dalam Menghambat Pertumbuhan Aeromonas salmonicida dan Vivrio harvey.E-jurnal Rekayasa dan Teknologi Budidaya Perairan. 1 (1): 2-8.

Vandepitte, S. 2005. Prosedur Laboratorium Dasar untuk Bakteriologis Klinis. Edisi 2. Buku Kedokteran EGC, Jakarta.

Refbacks

  • There are currently no refbacks.