Sensitivitas Bakteri Gram Negatif yang Diisolasi dari Kaki Kecoa (Periplaneta americana)di Area Rumah Sakit dan Perumahan di Kota Palu terhadapBeberapa Antibiotik

I Nengah Kundera, Mursito Bialangi

Abstract

Cockroaches (Periplaneta americana) are often found in homes, restaurants, hotels, hospitals, warehouses, offices, libraries, and others. The existence of these highly dynamic animals is suspected of causing nosocomial infections at the hospitals and acting as vectors and hosts associated with bacteria carried on its feet. This research aims to analyze the sensitivity of Gram-negative bacteria isolated from Cockroach feet in the hospital and residential areas in Palu City against some antibiotics. This research is an explorative laboratory experiment research, with the method of bacteria identification, isolation and antibiotic test using standard procedure "The Bauer-Kirby". The results of bacterial identification in three areas of the Palu Hospital are Klebsiella ozaenaeSalmoella arizonae, Salmonella sp. Escherichia coli, Salmonella cholerae-suis and Salmonella simultans, while the bacteria from the residential area, Escherichia coli, Klebsiellapneumoniae, Proteus vulgaris, Salmonella arizonae, and Proteus mirabilis. The results of bacterial sensitivity tests on five antibiotics showed that all of these bacteria were resistant to the antibiotics of Vancomycin, Chloramphenicol, and Amoxycillin, but were still sensitive to Ciprofloxacin and Ofloxacin.

Keywords

Cockroaches, bacteria, sensitivity, antibiotics

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References

Bouamama, L.; A. Sorlozano; A. Laglaoui; M. Lebbadi; A. Aarab & J. Gutierrez. 2010. Antibiotic resistance patterns of bacterial strains isolated from Periplaneta americanaand Muscadomestica in Tangier, Morocco. J.Infect. Dev. Ctries., 4: 194-201.

Cloarec A, Rivault C, Fontaine F, Le Guyader A.,(1992), Cockroaches as carriers of bacteria in multi-family dwellings. Epidemiol Infect. 109(3): 483-490.

Ejimadu, L. C., Goselle, O.N*., Ahmadu, Y. M. & James-Rugu, N.N., (2015). Specialization of Periplaneta Americana (American Cockroach) and Blattella Germanica (German cockroach) Towards Intestinal Parasites: A Public Health Concern, IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 10. (6): 23-32.

Kassiri, H & Shahnaz K., (2012), Cockroaches [Periplaneta americana (L.), Dictyoptera; Blattidae] as Carriers of Bacterial Pathogens, Khorramshahr County, Iran Jundishapur J Microbiol.5(1),320-322.

Kundera I N, P. Maharani A. A., & Evasari H.S. 2017. Identification of Bacteria on Cockroach Foot (Periplaneta americana) in Resident Bay of Palu Permai and Sensitivity Test Against Antibiotics. (Jurnal Biosaintifika, proses review).

Lamiaa B, Lebbadi M & Aarab A., (2007), Bacteriological analysis of Periplaneta americana L. (Dictyoptera; Blattidae) and Musca domestica L. (Diptera; Muscidae) in ten districts of Tangier, Morocco,Afr.J. Biotechnol,6(17),2038-2042.

Maharani A. A. P., Mursito B., &KunderaI.N., 2018. Identification of Bacteria on Cockroach Feet from Hospital area in Palu city and Test of Sensitivity to Antibiotic, ProsiddingThe 3rd Annual Applied Science and Engineering Conference (AASEC-2018) UPI, Bandung.

Mandell G.l., Bannett’s J.E & Dolin R, (2010) Principles and Practice of Infections Diseases,seven edition, Churchil Livingstone, Elsevier, Philadelphia.

Pai, H. H.; Chen, W. C. & Peng, C. F. (2004). Cockroaches as potential vectors of nosocomial infections.Infect. Control Hosp. Epidemiol., 25: 979-984.

Pechal J.L., James A., Roger.,& Jeffery K.T., 2007. Epidemiology and Spatial Relationships of Bacteria Associated withPeriplanetaamericana (Blattodea: Blattidae) in Central Texas, Journal of Agricultural and Urban Entomology 24(4):205-216.

Perilla, M. J., 2003. Manual for the Laboratory Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens of Public Health Importance in the Developing World, Centers for Disease Control and Prevention: National Center for Infectious Diseases, Atlanta, Georgia 30333 USA

Prado, M. A.; Gir, E.; Pereira, M. S. Reis, C. & Pimenta, F. C.(2006). Antimicrobial resistance of bacteria Isolated from Cockroaches (Periplaneta americana) in Brazilian Health Care Institution. Brazil. J. Infect. Dis., 10: 26-32.

Salehzadeh, A., Tavacol, P. & Mahjub, H. (2007). Bacteria, fungal and parasitic contamination of cockroaches in public hospitals of Hamadan, Iran.Journal of Vector-borne Diseases, 44: 105-110.

Subandi.(2010), Mikrobiologi (Perkembangan, KajianDan Pengamatan Dalam Perspektif Islam). Jakarta, Rosada.

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