KEBERADAAN BAKTERI PENGHASIL FITASE UNTUK PERBAIKAN KESUBURAN TANAH VERTISOL PADA BERBAGAI SISTEM BUDIDAYA TANAM DI KECAMATAN GONDANGREJO KABUPATEN KARANGANYAR

Slamet Santoso, SAJIDAN SAJIDAN

Abstract

This experiment aimed to investigate whether phytase-producing  bacteria isolated from vertisol soil in Gondangrejo have bacterial diversity and the ability to fertilize the soil. This research is exploratif and laboratory experiments. Bacteria in the rice roots (PJ2, PJ3, PP2, PP8, PU2, and PU +1) has the ability to grow at 70°C, and its enzyme activity is stable at 80°C and pH 4-8. Bacteria in the corn roots still grow (JP1, JW1, JPJ1, JPS1) and still active at 60°C, and the enzyme still active at 60°C and pH 4-8. Bacteria on the beans roots grew well (KPTU4, KPJW1, KPTU3, KTJ2) and still active at 60°C, and the enzyme activity at 60°C and pH 4-7. Bacteria on the sugar cane roots grew well (TS1, TS2, TU1, TJ4), active at 60°C and the enzyme activity at 60°C and pH 4-8. Bacteria on the cassava roots were good (SW2) and still active grow at 60°C, and at 90°C and pH 4-8, the enzyme still active. Bacteria on the great pumpkin roots were good (WP1, WP3), still active at 60°C, and the active enzyme
activity at 60°C and pH 5-8. Bacteria on the “gambas” roots grow well (GPP2, OU1, OU2) and still active at 60°C, and the enzyme still active at 60°C and pH 4-8. Rough of extracted enzyme from isolates of the best area of rice roots PU+2 and PU+1, beans KTJ2 and KPJW, sugar cane TU1 and TU2, corn JP1 and JPJ1, “gambas” GPP2 and OU2, were stable at 80°C. Selected isolates actived at pH 6-8 are PJ3, OU 2, WP1, WP4, JW1, JP1, KPtU 2, KJP1, KPtU4, TJ2, while PJ2 and TU1 were active in pH 6-9.

Keywords

Fitase; vertisol; kesuburan; pengaruh suhu; pengaruh pH

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